Core B is the central cell culture facility, which will provide support for each of four projects. This core facility will provide services for the following: 1) isolation of neutrophils (PMNs) from mouse blood and bone marrow;2) isolation, characterization, and culture of mouse lung endothelial cells, and 3) culture of human lung microvascular and pulmonary artery endothelial cells for the experiments involved in the Projects as outlined in the Core description below. Mouse lung endothelial cell isolation and culture is pivotal for the accomplishment of studies proposed in Projects 1, 2, 3, and 4. In addition. Core B will also isolate PMNs from mouse blood and bone marrow for Projeds 1, 2, and 3. A major task of this core will be to provide lung endothelial cells and PMNs from different knock-out mice;e.g., TRPM2 knock-out, NADPH oxidase subunit gp91(phox) knock-out, and PKC-alpha knock-out mice for Project 1;MKP5 knock-out, p47(phox) knock-out, MK2 knock-out, and MKP5/MK2-double knock-out mice for Project 2;TRPC4 knock-out;TRPM2 knock-out, and Dream knock-out mice for Project 3;FoxMI transgenic and iCKO mice for Project 4. Thus the adivity of this Core is essential for the success of the Program Projed studies.

Public Health Relevance

Core B, the Cell Culture Core, will provide endothelial cells and neutrophils to Projects 1 - 4 for their experiments designed to identify novel therapeutic targets for lung inflammatory diseases such as Acute Lung Injury.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Illinois at Chicago
United States
Zip Code
Evans, Colin E; Zhao, You-Yang (2017) Impact of thrombosis on pulmonary endothelial injury and repair following sepsis. Am J Physiol Lung Cell Mol Physiol 312:L441-L451
Du, Xueke; Jiang, Chunling; Lv, Yang et al. (2017) Isoflurane promotes phagocytosis of apoptotic neutrophils through AMPK-mediated ADAM17/Mer signaling. PLoS One 12:e0180213
Di, Anke; Kiya, Tomohiro; Gong, Haixia et al. (2017) Role of the phagosomal redox-sensitive TRP channel TRPM2 in regulating bactericidal activity of macrophages. J Cell Sci 130:735-744
Mittal, Manish; Nepal, Saroj; Tsukasaki, Yoshikazu et al. (2017) Response by Mittal et al to Letter Regarding Article, ""Neutrophil Activation of Endothelial Cell-Expressed TRPM2 Mediates Transendothelial Neutrophil Migration and Vascular Injury"". Circ Res 121:e87
Cheng, Kwong Tai; Xiong, Shiqin; Ye, Zhiming et al. (2017) Caspase-11-mediated endothelial pyroptosis underlies endotoxemia-induced lung injury. J Clin Invest 127:4124-4135
Reddy, Sekhar P; Mehta, Dolly (2017) Lung Interstitial Macrophages Redefined: It Is Not That Simple Anymore. Am J Respir Cell Mol Biol 57:135-136
Li, Liping; Sheng, Yue; Li, Wenshu et al. (2017) ?-Catenin Is a Candidate Therapeutic Target for Myeloid Neoplasms with del(5q). Cancer Res 77:4116-4126
Evans, Colin E; Zhao, You-Yang (2017) Molecular Basis of Nitrative Stress in the Pathogenesis of Pulmonary Hypertension. Adv Exp Med Biol 967:33-45
Tsang, Kit Man; Hyun, James S; Cheng, Kwong Tai et al. (2017) Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1?. Stem Cell Reports 9:796-806
Zhang, Chongxu; Adamos, Crystal; Oh, Myung-Jin et al. (2017) oxLDL induces endothelial cell proliferation via Rho/ROCK/Akt/p27kip1 signaling: opposite effects of oxLDL and cholesterol loading. Am J Physiol Cell Physiol 313:C340-C351

Showing the most recent 10 out of 99 publications