Core B is the central cell culture facility, which provides support for each of the three projects. The cell culture Core will perform the following functions and services: 1) isolate and culture alveolar and bone marrow-derived macrophages from wild type and genetically modified mice; 2) isolate and culture macrophages from human bronchoalveolar fluid; 3) isolate monocytes from human pheripheral blood; 4) Maintenance and propagation of primary human lung microvessel endothelial cells; 5) isolate, culture, and characterize lung endothelial cells from wild type and genetically modified mice; 6) isolate, culture, and characterize lung epithelial (Type I and Type II) cells from wild type and genetically modified mice; and 7) provide technical support for gene transcription regulation studies. Core B will supply alveolar and bone marrow- derived macrophages from wild type and knockout (Trpm2?/?, Nox2?/?, Nox4?/?, Cd38?/?, Trpm2Mye?/?, Nlrp3?/?, and caspase1?/?) mice, and human alveolar macrophages for the accomplishment of studies proposed in Project 1. For Project 2 studies, Core B will provide mouse lung endothelial and Type II epithelial cells, and supply human epithelial cells. In addition, Core B will also provide for studies in Project 2 bone marrow-derived macrophages from WT, Sphk2?/?, Par?/?, and Creb1Mye?/? mice. For studies proposed in Project 3, Core B will provide human monocytes and lung endothelial cells, lung endothelial cells from WT and Jag1i?EC mice, and alveolar and bone marrow-derived macrophages from wild type and knockout (Jag1i?EC, Notch1?Mye, Notch2?Mye, and Notch1-Notch2?Mye) mice. Additionally, Core B will provide technical support for analysis of gene transcription studies proposed in Projects 2 and 3. This Core is essential for the success of the Program Project.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL077806-14
Application #
9454533
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Program Officer
Aggarwal, Neil Raj
Project Start
2005-08-01
Project End
Budget Start
2018-04-01
Budget End
2019-03-31
Support Year
14
Fiscal Year
2018
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Type
DUNS #
098987217
City
Chicago
State
IL
Country
United States
Zip Code
60612
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