Genetically-modified mice provide a tractable approach to test the biological significance of protein function in complex processes such as thrombosis and leukocyte extravasation. Core A will serve as a mouse genetics core facility to provide advice, training, and services to project investigators in the design and implementation of mouse gene targeting and transgenesis projects including ES cell culture, transfection and screening for homologous recombination, blastocyst injection, and characterization of germline transmitted alleles in founder lines. In addition, Core A will provide project investigators with access to a library of mice maintained by the Core including Cre expressing lines for conditional deletion or overexpression of proteins in vascular cells (platelets, endothelial cells, smooth muscle cells, several types of leukocytes).

Agency
National Institute of Health (NIH)
Type
Research Program Projects (P01)
Project #
5P01HL078784-10
Application #
8666023
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92093
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