Development of genetically engineered mouse models is crucial to understanding the roles of specific genes in cardiac function and disease. The PPG makes extensive use of a variety of genetic approaches to study the role of several genes in cardiac function and disease. The purpose of the mouse core is to provide services for the generation and management of gene-targeted and transgenic mice. Founder mice will be identified, bred, and maintained in a Core animal facility at UCSD. The Core will also be responsible for maintaining and distributing mouse lines to each individual project. In addition, the Core will also cryopreserve critical lines in case of a disaster (e.g. natural disasters, infectious outbreaks, etc.).

Public Health Relevance

This PPG application is proposing many new studies with innovative experimental tools and genetically modified animal models, to answer significant fundamental mechanistic questions, as well as translational questions related to cardiac hypertrophy, failure and arrhythmia. The mouse core will provide services for the generation and management of gene-targeted and transgenic mice for all four projects in the PPG. Thus it is essential for the success of the PPG.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL080101-08
Application #
8496858
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
8
Fiscal Year
2013
Total Cost
$240,634
Indirect Cost
$84,378
Name
University of California Davis
Department
Type
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
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Bers, Donald M (2014) Cardiac sarcoplasmic reticulum calcium leak: basis and roles in cardiac dysfunction. Annu Rev Physiol 76:107-27
Xie, Yuanfang; Grandi, Eleonora; Bers, Donald M et al. (2014) How does ?-adrenergic signalling affect the transitions from ventricular tachycardia to ventricular fibrillation? Europace 16:452-7
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Zhang, Dai-Min; Chai, Yongping; Erickson, Jeffrey R et al. (2014) Intracellular signalling mechanism responsible for modulation of sarcolemmal ATP-sensitive potassium channels by nitric oxide in ventricular cardiomyocytes. J Physiol 592:971-90
Kapoor, Nidhi; Maxwell, Joshua T; Mignery, Gregory A et al. (2014) Spatially defined InsP3-mediated signaling in embryonic stem cell-derived cardiomyocytes. PLoS One 9:e83715
Morotti, S; Edwards, A G; McCulloch, A D et al. (2014) A novel computational model of mouse myocyte electrophysiology to assess the synergy between Na+ loading and CaMKII. J Physiol 592:1181-97

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