For the individual projects, gene expression analyses will be provided for specific cellular, animal, and clinical phenotypes. Importantly, this core lab will develop custom targeted gene expression and miRNA arrays that will provide the capacity to examine and directly compare related gene sets involved in endothelial function, mitochondrial biogenesis and function, reactive oxygen species, adipocytes, macrophages and hypoxia/angiogenesis. The Gene Expression Core has previously collaborated or published with Drs. Gokce and Vita and has begun studying miRNA and high-throughput gene expression with Drs. Cohen and Walsh. The Gene Expression Core Laboratory is an established facility with automated robotic pipeting using computerized programs, automated RNA isolation, storage, and custom chip capacity. The Laboratory is already involved in high-throughput analysis of over 10,000 subjects'gene expression, miRNA, and protein samples from various clinical projects. The laboratory has also assisted investigators in gene expression analysis from small volume tissue using murine and cell culture models. Importantly, there will be developed an established panel of genes and miRNA that will be compared across studies to determine the mechanistic overlap between specific inflammatory states and metabolic diseases and the role individual cell types play in endothelial and vascular disease (see Table 1).

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL081587-08
Application #
8438335
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-03-01
Budget End
2014-02-28
Support Year
8
Fiscal Year
2013
Total Cost
$209,525
Indirect Cost
$81,531
Name
Boston University
Department
Type
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118
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Romero, Freddy; Shah, Dilip; Duong, Michelle et al. (2015) A pneumocyte-macrophage paracrine lipid axis drives the lung toward fibrosis. Am J Respir Cell Mol Biol 53:74-86
Kikuchi, Ryosuke; Nakamura, Kazuto; MacLauchlan, Susan et al. (2014) An antiangiogenic isoform of VEGF-A contributes to impaired vascularization in peripheral artery disease. Nat Med 20:1464-71
Ngo, Doan T M; Farb, Melissa G; Kikuchi, Ryosuke et al. (2014) Antiangiogenic actions of vascular endothelial growth factor-A165b, an inhibitory isoform of vascular endothelial growth factor-A, in human obesity. Circulation 130:1072-80
Tan, Peng H; Tyrrell, Helen E J; Gao, Liquan et al. (2014) Adiponectin receptor signaling on dendritic cells blunts antitumor immunity. Cancer Res 74:5711-22
Hartman, Mor-Li; Shirihai, Orian S; Holbrook, Monika et al. (2014) Relation of mitochondrial oxygen consumption in peripheral blood mononuclear cells to vascular function in type 2 diabetes mellitus. Vasc Med 19:67-74
Nakamura, Kazuto; Fuster, José J; Walsh, Kenneth (2014) Adipokines: a link between obesity and cardiovascular disease. J Cardiol 63:250-9
Shimizu, Ippei; Aprahamian, Tamar; Kikuchi, Ryosuke et al. (2014) Vascular rarefaction mediates whitening of brown fat in obesity. J Clin Invest 124:2099-112
Yoshida, Sumiko; Fuster, José Javier; Walsh, Kenneth (2014) Adiponectin attenuates abdominal aortic aneurysm formation in hyperlipidemic mice. Atherosclerosis 235:339-46
Parker-Duffen, Jennifer L; Walsh, Kenneth (2014) Cardiometabolic effects of adiponectin. Best Pract Res Clin Endocrinol Metab 28:81-91

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