The purpose of the Neuroanatomy and Neurophysiology core will be to provide expertise, technical assistance and centralized equipment and facilities for all neuroanatomical and sympathetic nerve recording aspects of the program project experiments. The facilities available will allow investigators to access equipment and materials necessary for preparation of the high quality microscopic material and state-of-the-art technique to study sympathetic nervous system activity, all essential for successful completion and interpretation of experiments performed on the brain. As a new addition, a subcontract of the core located at Weill Cornell Medical College will perform ER stress biomarker analysis. This will be achieved in a cost-effective manner by the centralization of facilities and expertise. Specifically, the core will provide: i) Expertise and instrumentation for gene transfer into the mouse brain nuclei in highly specific manner;ii) A full service for the preparation of morphological and immunocytochemical material for analysis with light and electron microscopy. This will include fixation, thin and ultrathin sectioning, and pre-embedding immunocytochemistry, etc. Expertise in the analysis and interpretation of this material will be provided;iii) Quantitative methods for determining cell numbers, etc., and planning, designing and implementing neuroanatomical experiments;iv) Expertise and instrumentation for sympathetic nerve recording, including data analysis and interpretation;v) Training in experimental neuroanatomy and neurophysiology for principal and co-investigators and associated postdoctoral and other students;vi) Measurements of ER stress biomarkers in brain micropunches harvested for Projects 2 and 3 by this Core B at Ul, and harvested at Cornell by Project 1. The core will perform experiments involving a) the precise targeting of brain regions with adenoviral vectors b) the identification of specific neuronal populations in complex brain regions through the use of antibodies and other markers;c) the use of electron microscopic methods to determine the presence of morphological changes associated with ER stress, the interactions of neural elements, and the subcellular localization of antigens not resolvable with light microscopy, d) nerve recordings from sympathetic and other afferent fibers innervating the kidney, muscle and adipose tissue, and e) measures of ER stress biomarkers.

Public Health Relevance

Core B mission is to facilitate research carried out by the entire PPG and to accelerate discovery by providing a seamless pipeline for the analysis of neuroanatomical features in mice, direct measurement of sympathetic nervous system activity subserving various beds, and measurements of endoplasmic reticulum stress.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Special Emphasis Panel (ZHL1-PPG-J (F1))
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University of Iowa
Iowa City
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Shinohara, Keisuke; Nakagawa, Pablo; Gomez, Javier et al. (2017) Selective Deletion of Renin-b in the Brain Alters Drinking and Metabolism. Hypertension 70:990-997
Contreras, Cristina; Nogueiras, Rubén; Diéguez, Carlos et al. (2017) Traveling from the hypothalamus to the adipose tissue: The thermogenic pathway. Redox Biol 12:854-863
Jiang, Jingwei; Cui, Huxing; Rahmouni, Kamal (2017) Optogenetics and pharmacogenetics: principles and applications. Am J Physiol Regul Integr Comp Physiol 313:R633-R645
Martínez-Sánchez, Noelia; Seoane-Collazo, Patricia; Contreras, Cristina et al. (2017) Hypothalamic AMPK-ER Stress-JNK1 Axis Mediates the Central Actions of Thyroid Hormones on Energy Balance. Cell Metab 26:212-229.e12
Grobe, Justin L (2017) Comprehensive Assessments of Energy Balance in Mice. Methods Mol Biol 1614:123-146
Agassandian, Khristofor; Grobe, Justin L; Liu, Xuebo et al. (2017) Evidence for intraventricular secretion of angiotensinogen and angiotensin by the subfornical organ using transgenic mice. Am J Physiol Regul Integr Comp Physiol 312:R973-R981
Sapouckey, Sarah A; Deng, Guorui; Sigmund, Curt D et al. (2017) Potential mechanisms of hypothalamic renin-angiotensin system activation by leptin and DOCA-salt for the control of resting metabolism. Physiol Genomics 49:722-732
Srisai, Dollada; Yin, Terry C; Lee, Abigail A et al. (2017) MRAP2 regulates ghrelin receptor signaling and hunger sensing. Nat Commun 8:713
Chhabra, Kavaljit H; Morgan, Donald A; Tooke, Benjamin P et al. (2017) Reduced renal sympathetic nerve activity contributes to elevated glycosuria and improved glucose tolerance in hypothalamus-specific Pomc knockout mice. Mol Metab 6:1274-1285
Riedl, Ruth A; Atkinson, Samantha N; Burnett, Colin M L et al. (2017) The Gut Microbiome, Energy Homeostasis, and Implications for Hypertension. Curr Hypertens Rep 19:27

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