The Physiology / Surgery Core (PSC;Core C), used by all of the Projects of this PPG, will offer integrated services for assessing heart function and structure in settings that mimic cardiac pathology in mice. Accordingly, Core C will maintain equipment and provide staffing to offer Projects the following services: 1) Physiology: echocardiography, invasive hemodynamics, isolated working heart preparation, and 2) Surgery: in vivo myocardial infarction, reperfusion, transaortic constriction, and intramyocardial adoptive transfer cell injection. The PSC is centrally located in the SDSU BioScience Center, which houses the SDSU Heart Institute labs and administrative offices. The PSC is composed of fully equipped surgery and physiology suites and dedicated mouse holding rooms. PPG investigators both institutions will be able to fully utilize PSC resources with """"""""on demand"""""""" availability of two part-time trained cardiac surgeons, a physiology technician, and a mouse husbandry specialist. All required equipment including a Visualsonics Vevo 770 and Scisense hemodynamic assessment package are in place and operational in the PSC. The PSC is under the direction of Dr. Mark Sussman, who has a legacy of publications in the techniques offered by core and is well versed in administration of such services for Program participants. Dr. Glembotski brings his decades of experience in mouse physiology and surgery as co-Director of the PSC. An additional advantage of the PSC is that it adjoins the SDSU Heart Institute Mouse Genetics Center, which facilitates the generation and use of genetically modified mice by Projects. The PSC was designed specifically to serve this multi-institutional PPG;accordingly, it will maximize collaborations between projects, thus enhancing the synergy of this integrated research program. The PSC complements and integrates with the Cell Biology / Histology Core (CBHC) by serving as the location where principles discovered using cells generated with cells from the CBHC will be assessed in a pathophysiological in vivo context that mimics cardiomyopathic conditions. The PSC will be equally used by all four Projects of the Program.

Public Health Relevance

The Program is designed to assess mechanisms to restore myocardial healing in the damaged heart, which can only be property tested with an in vivo model system that allows for creation of pathophysiological damage that mimics the human disease conditions of hypertrophy and infarction. This core facility provides both the capabilities to create such models in mice and then evaluate the ability of the heart to recover.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
San Diego State University
San Diego
United States
Zip Code
Reynolds, Julia O; Quick, Ann P; Wang, Qiongling et al. (2016) Junctophilin-2 gene therapy rescues heart failure by normalizing RyR2-mediated Ca(2+) release. Int J Cardiol 225:371-380
Shirakabe, Akihiro; Fritzky, Luke; Saito, Toshiro et al. (2016) Evaluating mitochondrial autophagy in the mouse heart. J Mol Cell Cardiol 92:134-9
Miyamoto, Shigeki; Brown, Joan Heller (2016) Drp1 and Mitochondrial Autophagy Lend a Helping Hand in Adaptation to Pressure Overload. Circulation 133:1225-7
Castaldi, Alessandra; Chesini, Gino P; Taylor, Amy E et al. (2016) Sphingosine 1-phosphate elicits RhoA-dependent proliferation and MRTF-A mediated gene induction in CPCs. Cell Signal 28:871-9
Broughton, Kathleen M; Sussman, Mark A (2016) Empowering Adult Stem Cells for Myocardial Regeneration V2.0: Success in Small Steps. Circ Res 118:867-80
Yu, Olivia M; Miyamoto, Shigeki; Brown, Joan Heller (2016) Myocardin-Related Transcription Factor A and Yes-Associated Protein Exert Dual Control in G Protein-Coupled Receptor- and RhoA-Mediated Transcriptional Regulation and Cell Proliferation. Mol Cell Biol 36:39-49
Leon, Leonardo J; Gustafsson, Ã…sa B (2016) Staying young at heart: autophagy and adaptation to cardiac aging. J Mol Cell Cardiol 95:78-85
Samse, Kaitlen; Hariharan, Nirmala; Sussman, Mark A (2016) Personalizing cardiac regenerative therapy: At the heart of Pim1 kinase. Pharmacol Res 103:13-6
Jin, Jung-Kang; Blackwood, Erik A; Azizi, Khalid M et al. (2016) ATF6 Decreases Myocardial Ischemia/Reperfusion Damage and Links ER Stress and Oxidative Stress Signaling Pathways in the Heart. Circ Res :
Shires, Sarah E; Gustafsson, Ã…sa B (2015) Mitophagy and heart failure. J Mol Med (Berl) 93:253-62

Showing the most recent 10 out of 134 publications