The Physiology / Surgery Core (PSC;Core C), used by all of the Projects of this PPG, will offer integrated services for assessing heart function and structure in settings that mimic cardiac pathology in mice. Accordingly, Core C will maintain equipment and provide staffing to offer Projects the following services: 1) Physiology: echocardiography, invasive hemodynamics, isolated working heart preparation, and 2) Surgery: in vivo myocardial infarction, reperfusion, transaortic constriction, and intramyocardial adoptive transfer cell injection. The PSC is centrally located in the SDSU BioScience Center, which houses the SDSU Heart Institute labs and administrative offices. The PSC is composed of fully equipped surgery and physiology suites and dedicated mouse holding rooms. PPG investigators both institutions will be able to fully utilize PSC resources with """"""""on demand"""""""" availability of two part-time trained cardiac surgeons, a physiology technician, and a mouse husbandry specialist. All required equipment including a Visualsonics Vevo 770 and Scisense hemodynamic assessment package are in place and operational in the PSC. The PSC is under the direction of Dr. Mark Sussman, who has a legacy of publications in the techniques offered by core and is well versed in administration of such services for Program participants. Dr. Glembotski brings his decades of experience in mouse physiology and surgery as co-Director of the PSC. An additional advantage of the PSC is that it adjoins the SDSU Heart Institute Mouse Genetics Center, which facilitates the generation and use of genetically modified mice by Projects. The PSC was designed specifically to serve this multi-institutional PPG;accordingly, it will maximize collaborations between projects, thus enhancing the synergy of this integrated research program. The PSC complements and integrates with the Cell Biology / Histology Core (CBHC) by serving as the location where principles discovered using cells generated with cells from the CBHC will be assessed in a pathophysiological in vivo context that mimics cardiomyopathic conditions. The PSC will be equally used by all four Projects of the Program.

Public Health Relevance

The Program is designed to assess mechanisms to restore myocardial healing in the damaged heart, which can only be property tested with an in vivo model system that allows for creation of pathophysiological damage that mimics the human disease conditions of hypertrophy and infarction. This core facility provides both the capabilities to create such models in mice and then evaluate the ability of the heart to recover.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL085577-07
Application #
8734481
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
7
Fiscal Year
2014
Total Cost
$220,401
Indirect Cost
$72,975
Name
San Diego State University
Department
Type
DUNS #
073371346
City
San Diego
State
CA
Country
United States
Zip Code
92182
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Shires, Sarah E; Kitsis, Richard N; Gustafsson, Åsa B (2017) Beyond Mitophagy: The Diversity and Complexity of Parkin Function. Circ Res 120:1234-1236
Monsanto, Megan M; Wang, Bingyan J; Sussman, Mark A (2017) Synthetic MSC? Nothing Beats the Real Thing. Circ Res 120:1694-1695
Khalafalla, Farid G; Greene, Steven; Khan, Hashim et al. (2017) P2Y2 Nucleotide Receptor Prompts Human Cardiac Progenitor Cell Activation by Modulating Hippo Signaling. Circ Res 121:1224-1236
Kubli, Dieter A; Sussman, Mark A (2017) Eat, breathe, ROS: controlling stem cell fate through metabolism. Expert Rev Cardiovasc Ther 15:345-356
Monsanto, Megan M; White, Kevin S; Kim, Taeyong et al. (2017) Concurrent Isolation of 3 Distinct Cardiac Stem Cell Populations From a Single Human Heart Biopsy. Circ Res 121:113-124
Glembotski, Christopher C (2017) Expanding the Paracrine Hypothesis of Stem Cell-Mediated Repair in the Heart: When the Unconventional Becomes Conventional. Circ Res 120:772-774
Sussman, Mark A (2017) A Matter of Opinion. Circ Res 120:36-38
Gray, Charles B B; Suetomi, Takeshi; Xiang, Sunny et al. (2017) CaMKII? subtypes differentially regulate infarct formation following ex vivo myocardial ischemia/reperfusion through NF-?B and TNF-?. J Mol Cell Cardiol 103:48-55
Giricz, Zoltán; Varga, Zoltán V; Koncsos, Gábor et al. (2017) Autophagosome formation is required for cardioprotection by chloramphenicol. Life Sci 186:11-16

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