The Morphology and Imaging Core will provide for analyses of atherosclerosis in animal models, providing measures of extent, rate of progression and composition using both conventional and novel technologies. In addition it will provide a variety of imaging techniques useful in both in vitro and in vivo analyses of experimental models. This Core will be a collaborative effort between units located at The Scripps Research Institute under the direction of Dr. Linda Curtiss and one located at University California, San Diego under Dr. Yury Miller. The overall goal of the Core will be to provide investigators in each of the units both conventional and novel techniques to assess both the extent as well as progression of atherosclerosis in murine models, to provide techniques to allow characterization of lesions, and to provide a variety of imaging techniques useful in both in vitro and in vivo experimental models.
The Aims of this Core are:
Specific Aim 1 : To provide measurements of the extent of atherosclerosis in murine models. This will include use of standard en face measurements of areas throughout the length of the aorta, the use of a computer assisted technique to provide more standardized volume measurements of lesion burden at the aortic sinus, and use of a novel measurement of rate of early lesion progression using laser scanning confocal immunofluorescence to provide a quantitative technique to asses in situ aortic macrophage infiltration.
Specific Aim 2 : To provide qualitative and quantitative imaging analysis of tissue and cellular morphology. This will include use of laser scanning confocal microscopy studies to provide novel qualitative images, in three dimension, of lesions at various stages of development, as well as the use of deconvolution microscopy to provide qualitative and quantitative analysis of morphology within lesions or cells in culture.
Specific Aim 3 : To validate the use of laser capture microdissection to allow microanalysis of gene expression in lesions or within groups of defined cells within lesions.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL088093-05
Application #
8375400
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
2014-03-31
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
5
Fiscal Year
2012
Total Cost
$282,955
Indirect Cost
$45,573
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Sherman, Mara H; Yu, Ruth T; Tseng, Tiffany W et al. (2017) Stromal cues regulate the pancreatic cancer epigenome and metabolome. Proc Natl Acad Sci U S A 114:1129-1134
Fan, Weiwei; Evans, Ronald M (2017) Exercise Mimetics: Impact on Health and Performance. Cell Metab 25:242-247
Choi, Soo-Ho; Sviridov, Dmitri; Miller, Yury I (2017) Oxidized cholesteryl esters and inflammation. Biochim Biophys Acta 1862:393-397
He, Nanhai; Fan, Weiwei; Henriquez, Brian et al. (2017) Metabolic control of regulatory T cell (Treg) survival and function by Lkb1. Proc Natl Acad Sci U S A 114:12542-12547
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van Capelleveen, Julian C; Bernelot Moens, Sophie J; Yang, Xiaohong et al. (2017) Apolipoprotein C-III Levels and Incident Coronary Artery Disease Risk: The EPIC-Norfolk Prospective Population Study. Arterioscler Thromb Vasc Biol 37:1206-1212
Fan, Weiwei; Waizenegger, Wanda; Lin, Chun Shi et al. (2017) PPAR? Promotes Running Endurance by Preserving Glucose. Cell Metab 25:1186-1193.e4
Doktorova, Marcela; Zwarts, Irene; Zutphen, Tim van et al. (2017) Intestinal PPAR? protects against diet-induced obesity, insulin resistance and dyslipidemia. Sci Rep 7:846
Wang, Jianrong; He, Nanhai; Zhang, Na et al. (2017) NCoR1 restrains thymic negative selection by repressing Bim expression to spare thymocytes undergoing positive selection. Nat Commun 8:959
Liu, Chao; Gaudet, Daniel; Miller, Yury I (2017) Deficient Cholesterol Esterification in Plasma of apoc2 Knockout Zebrafish and Familial Chylomicronemia Patients. PLoS One 12:e0169939

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