The Genomics and Bioinformatics Core will provide resources and technical and bioinformatics support for the application of massively parallel sequencing-based technologies to the understanding of regulated gene expression in macrophages, T cells, B cells and endothelial cells. The Genomics and Bioinformatics Core will thereby enable the acquisition and sophisticated analysis of data generated by ChlP-Seq, RNA-Seq, GRO-Seq and Ribo-Seq experiments. These methods provide extremely powerful approaches to addressing key mechanistic and pathophysiologic questions by each ofthe four projects ofthe PPG.
The ability to globally evaluate gene expression and transcription factor location using massively parallel DNA sequencing approaches provides a relatively unbiased approach to determining functional roles of these factors and upstream signaling pathways in regulation of cellular phenotypes. This information is likely to provide new insights into how specific programs of gene expression in macrophages, T cells, B cells and endothelial cells are altered in atherosclerosis.
|Fan, Weiwei; Evans, Ronald (2015) PPARs and ERRs: molecular mediators of mitochondrial metabolism. Curr Opin Cell Biol 33:49-54|
|Tsimikas, Sotirios; Duff, Gordon W; Berger, Peter B et al. (2014) Pro-inflammatory interleukin-1 genotypes potentiate the risk of coronary artery disease and cardiovascular events mediated by oxidized phospholipids and lipoprotein(a). J Am Coll Cardiol 63:1724-34|
|Gonen, Ayelet; Hansen, Lotte F; Turner, William W et al. (2014) Atheroprotective immunization with malondialdehyde-modified LDL is hapten specific and dependent on advanced MDA adducts: implications for development of an atheroprotective vaccine. J Lipid Res 55:2137-55|
|Baldan, Angel; Gonen, Ayelet; Choung, Christina et al. (2014) ABCG1 is required for pulmonary B-1 B cell and natural antibody homeostasis. J Immunol 193:5637-48|
|Daniel, Bence; Nagy, Gergely; Hah, Nasun et al. (2014) The active enhancer network operated by liganded RXR supports angiogenic activity in macrophages. Genes Dev 28:1562-77|
|Hilgendorf, Ingo; Theurl, Igor; Gerhardt, Louisa M S et al. (2014) Innate response activator B cells aggravate atherosclerosis by stimulating T helper-1 adaptive immunity. Circulation 129:1677-87|
|d'Uscio, Livius V; He, Tongrong; Santhanam, Anantha Vijay R et al. (2014) Mechanisms of vascular dysfunction in mice with endothelium-specific deletion of the PPAR-? gene. Am J Physiol Heart Circ Physiol 306:H1001-10|
|Hong, Suk-Hyun; Ahmadian, Maryam; Yu, Ruth T et al. (2014) Nuclear receptors and metabolism: from feast to famine. Diabetologia 57:860-7|
|Suh, Jae Myoung; Jonker, Johan W; Ahmadian, Maryam et al. (2014) Endocrinization of FGF1 produces a neomorphic and potent insulin sensitizer. Nature 513:436-9|
|Zhao, Xuan; Cho, Han; Yu, Ruth T et al. (2014) Nuclear receptors rock around the clock. EMBO Rep 15:518-28|
Showing the most recent 10 out of 51 publications