The Morphology Unit and Imaging Core will provide for analyses of atherosclerosis in animal models, providing measures of extent, rate of progression and composition using both conventional and novel techniques. For this PPG renewal, the Morphology Core will be consolidated in one location, at UCSD under direction of Dr. Yury Miller. The overall goal will be to provide Investigators in the Projects both conventional and novel techniques to assess atherosclerosis. The 1st Specific Aim is to provide quantitative measurements of the extent of atherosclerosis in animal models. This includes conventional analysis of en face lesions, lesion volume at the root ofthe aorta, and recently, lesion volume in the abdominal aorta. The 2nd Specific Aim is to provide quantitation of macrophage infiltration in atherosclerotic lesions by use of laser scanning confocal microscopy and immunofluorescent techniques, including those previously developed by Dr. Curtiss. The 3rd Specific Aim will provide qualitative and quantitative imaging analysis of tissue and cellular morphology. This includes the provision of non-fixed tissues for gene expression studies, paraffin embedded or frozen sections for lesion or cellular morphology, and also includes laser capture microdissection as needed.
Specific Aim 4 will provide dissected peri-aortic adipose tissue from the thoracic and abdominal aortas. This is a new function that will meet the new focus on peri-aortic adipose tissue that will be a major area of study for Project 2, and to a lesser extent in Project 3. The Morphology and Imaging Core is widely used by Investigators ofthe PPG and has evolved to meet new needs as noted. We anticipate that it will continue to provide a centrally important role in the life ofthe PPG.

Public Health Relevance

The Morphology and Imaging Core provide Investigators a way to measure the extent of atherosclerosis in animal models and the opportunity to learn how to do interventions that can reduce the extent of disease or even prevent it from occurring in the first place.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California San Diego
La Jolla
United States
Zip Code
Sherman, Mara H; Yu, Ruth T; Tseng, Tiffany W et al. (2017) Stromal cues regulate the pancreatic cancer epigenome and metabolome. Proc Natl Acad Sci U S A 114:1129-1134
Fan, Weiwei; Evans, Ronald M (2017) Exercise Mimetics: Impact on Health and Performance. Cell Metab 25:242-247
Choi, Soo-Ho; Sviridov, Dmitri; Miller, Yury I (2017) Oxidized cholesteryl esters and inflammation. Biochim Biophys Acta 1862:393-397
He, Nanhai; Fan, Weiwei; Henriquez, Brian et al. (2017) Metabolic control of regulatory T cell (Treg) survival and function by Lkb1. Proc Natl Acad Sci U S A 114:12542-12547
Shalapour, Shabnam; Lin, Xue-Jia; Bastian, Ingmar N et al. (2017) Inflammation-induced IgA+ cells dismantle anti-liver cancer immunity. Nature 551:340-345
van Capelleveen, Julian C; Bernelot Moens, Sophie J; Yang, Xiaohong et al. (2017) Apolipoprotein C-III Levels and Incident Coronary Artery Disease Risk: The EPIC-Norfolk Prospective Population Study. Arterioscler Thromb Vasc Biol 37:1206-1212
Fan, Weiwei; Waizenegger, Wanda; Lin, Chun Shi et al. (2017) PPAR? Promotes Running Endurance by Preserving Glucose. Cell Metab 25:1186-1193.e4
Doktorova, Marcela; Zwarts, Irene; Zutphen, Tim van et al. (2017) Intestinal PPAR? protects against diet-induced obesity, insulin resistance and dyslipidemia. Sci Rep 7:846
Wang, Jianrong; He, Nanhai; Zhang, Na et al. (2017) NCoR1 restrains thymic negative selection by repressing Bim expression to spare thymocytes undergoing positive selection. Nat Commun 8:959
Liu, Chao; Gaudet, Daniel; Miller, Yury I (2017) Deficient Cholesterol Esterification in Plasma of apoc2 Knockout Zebrafish and Familial Chylomicronemia Patients. PLoS One 12:e0169939

Showing the most recent 10 out of 144 publications