The purpose of this core is to provide aortic flow cytometry and fluorescence-activated cell sorting services to all three projects. The Pi's lab developed flow cytometry for mouse aortas. Based on fully developed and validated technology (more than 45 markers have been validated, including transcription factors and intracellular cytokines), the core will analyze myeloid cells (neutrophils, monocytes, macrophages, DCs), and lymphocytes (T cell subsets, B1a, B1b, B2) in the wall of aortas of wild-type C57BL/6, Apoe-/- or Ldir-/- mice. We will also analyze the leukocyte content in periarterial adipose tissue, in paraaortic lymph nodes, in distant lymph nodes and in the spleen. There are three specific aims: 1. To provide leukocyte phenotyping and sorting of mouse aortas to all projects;2. To provide separate leukocyte phenotyping in intima and media, adventitia, and periaortic adipose tissue;3. To provide leukocyte phenotyping in paraaortic (draining) lymph nodes, other lymph nodes and spleen. The key procedure is obtaining a single cell suspension from mouse aortas, with a high level of viability (>70%) and nearly complete extraction. This procedure is done in each ofthe projects, so no live animals will be used in the core. An experienced technician will train postdocs and technicians from the three projects in harvesting. During the enzymatic digestion step, the aortas will be transported to LlAl in a thermally controlled container. All labs are local and within a few minutes of each other. The actual flow cytometry by FACSCalibur or LSR-II (Becton Dickinson) will be done at LlAl.

National Institute of Health (NIH)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California San Diego
La Jolla
United States
Zip Code
Fan, Weiwei; Evans, Ronald (2015) PPARs and ERRs: molecular mediators of mitochondrial metabolism. Curr Opin Cell Biol 33:49-54
Tsimikas, Sotirios; Duff, Gordon W; Berger, Peter B et al. (2014) Pro-inflammatory interleukin-1 genotypes potentiate the risk of coronary artery disease and cardiovascular events mediated by oxidized phospholipids and lipoprotein(a). J Am Coll Cardiol 63:1724-34
Gonen, Ayelet; Hansen, Lotte F; Turner, William W et al. (2014) Atheroprotective immunization with malondialdehyde-modified LDL is hapten specific and dependent on advanced MDA adducts: implications for development of an atheroprotective vaccine. J Lipid Res 55:2137-55
Baldan, Angel; Gonen, Ayelet; Choung, Christina et al. (2014) ABCG1 is required for pulmonary B-1 B cell and natural antibody homeostasis. J Immunol 193:5637-48
Daniel, Bence; Nagy, Gergely; Hah, Nasun et al. (2014) The active enhancer network operated by liganded RXR supports angiogenic activity in macrophages. Genes Dev 28:1562-77
Hilgendorf, Ingo; Theurl, Igor; Gerhardt, Louisa M S et al. (2014) Innate response activator B cells aggravate atherosclerosis by stimulating T helper-1 adaptive immunity. Circulation 129:1677-87
d'Uscio, Livius V; He, Tongrong; Santhanam, Anantha Vijay R et al. (2014) Mechanisms of vascular dysfunction in mice with endothelium-specific deletion of the PPAR-? gene. Am J Physiol Heart Circ Physiol 306:H1001-10
Hong, Suk-Hyun; Ahmadian, Maryam; Yu, Ruth T et al. (2014) Nuclear receptors and metabolism: from feast to famine. Diabetologia 57:860-7
Suh, Jae Myoung; Jonker, Johan W; Ahmadian, Maryam et al. (2014) Endocrinization of FGF1 produces a neomorphic and potent insulin sensitizer. Nature 513:436-9
Zhao, Xuan; Cho, Han; Yu, Ruth T et al. (2014) Nuclear receptors rock around the clock. EMBO Rep 15:518-28

Showing the most recent 10 out of 51 publications