The overall goals ofthe program project grant (PPG) and individual projects require primary human cells from the two human subjects protocols described in Core A (Clinical Core). The goal of Core B (Laboratory Core) is to process the clinical samples from the human subjects protocols to provide high quality cells to each project for planned in vitro experiments and future analysis of clinical samples. Specific Task 1: Isolate purified eosinophils (EOS) and other cell populations from peripheral blood donors. Subjects that have been well characterized by Core A (Clinical Core) for the blood donor protocol will be recruited for peripheral blood donation. Using gradient centrifugation and magnetic bead negative separation techniques, purified EOS and/or other cell types (e.g. monocytes and CD4^ T cells) will be prepared for in vitro experimental use by the PPG projects. Spcicific Task 2: Process bronchoalveolar lavage (BAL) samples to isolate EOS and other cell populations. BAL fluid derived from subjects enrolled in the Core A (Clinical Core) protocol will be collected. Using gradient centrifugation and other cell separation techniques, purified EOS and/or other cell types (e.g. monocytes and T cells) will be prepared for in vitro experimental use by the PPG projects. Specific Task 3: Adapt current protocols to the requirements of each project. Any ofthe protocols in the first two tasks and other EOS functional assays will be adapted for the needs of any project.

Public Health Relevance

The relevance ofthe Laboratory Core is to provide critical and high quality purified EOS from blood and BAL as well as monocytes, 004"" T cells, and neutrophils from blood to the three projects to interrogate the role of EOS in airway remodeling associated with asthma.

National Institute of Health (NIH)
Research Program Projects (P01)
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Heart, Lung, and Blood Program Project Review Committee (HLBP)
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University of Wisconsin Madison
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Schwantes, E A; Manthei, D M; Denlinger, L C et al. (2014) Interferon gene expression in sputum cells correlates with the Asthma Index Score during virus-induced exacerbations. Clin Exp Allergy 44:813-21
Esnault, Stephane; Kelly, Elizabeth A; Johansson, Mats W et al. (2014) Semaphorin 7A is expressed on airway eosinophils and upregulated by IL-5 family cytokines. Clin Immunol 150:90-100
Wickert, Lisa E; Karta, Maya R; Audhya, Anjon et al. (2014) Simvastatin attenuates rhinovirus-induced interferon and CXCL10 secretion from monocytic cells in vitro. J Leukoc Biol 95:951-9
Karta, Maya R; Gavala, Monica L; Curran, Colleen S et al. (2014) LPS modulates rhinovirus-induced chemokine secretion in monocytes and macrophages. Am J Respir Cell Mol Biol 51:125-34
Han, Shih-Tsung; Mosher, Deane F (2014) IL-5 induces suspended eosinophils to undergo unique global reorganization associated with priming. Am J Respir Cell Mol Biol 50:654-64
Mathur, Sameer K; Viswanathan, Ravi K (2014) Relevance of allergy in adult asthma. Curr Allergy Asthma Rep 14:437
Burnham, Mandy E; Esnault, Stephane; Roti Roti, Elon C et al. (2014) Cholesterol selectively regulates IL-5 induced mitogen activated protein kinase signaling in human eosinophils. PLoS One 9:e103122
Karta, Maya R; Wickert, Lisa E; Curran, Colleen S et al. (2014) Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages. J Allergy Clin Immunol 133:1227-30
Johansson, M W (2014) Activation states of blood eosinophils in asthma. Clin Exp Allergy 44:482-98
Oh, Jiyoung; Malter, James S (2013) Pin1-FADD interactions regulate Fas-mediated apoptosis in activated eosinophils. J Immunol 190:4937-45

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