The Histology &Imaging Core will provide a central site and umbrella authority, which together with satellite infrastructure on this campus confers necessary services, instrumentation, supervision and training for PPG members and personnel within their laboratories. The Core's two major functions are (i) to assist characterization of undifferentiated and differentiated phenotypes of mouse and human embryonic stem cells and their derivatives (all Subprojects), and (2) to assist with the unambiguous histologic characterization of engrafted and regenerated myocardium following stem cell transplantation (Subprojects 1, 3, 4). To achieve these goals the core will assume a training and advisory role while providing services including fixation, embedding and sectioning;routine, histochemical and immunohistochemical staining;widefield and fluorescence microscopy;laser-scanning confocal and multi-photon microscopy;electron microscopy; microangiography. By maintaining an organized online database, processing of images to reconstruct and qualitatively analyze the extent of stem cell engraftment in transplanted myocardium will be provided.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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Medical College of Wisconsin
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Kolander, Kurt D; Holtz, Mary L; Cossette, Stephanie M et al. (2014) Epicardial GATA factors regulate early coronary vascular plexus formation. Dev Biol 386:204-15
Lakshmikanthan, Sribalaji; Zieba, Bartosz J; Ge, Zhi-Dong et al. (2014) Rap1b in smooth muscle and endothelium is required for maintenance of vascular tone and normal blood pressure. Arterioscler Thromb Vasc Biol 34:1486-94
Fisher, Joseph B; Kim, Min-Su; Blinka, Steven et al. (2012) Stress-induced cell-cycle activation in Tip60 haploinsufficient adult cardiomyocytes. PLoS One 7:e31569
Van Orman, Jordan R; Si-Tayeb, Karim; Duncan, Stephen A et al. (2012) Induction of cardiomyogenesis in human embryonic stem cells by human embryonic stem cell-derived definitive endoderm. Stem Cells Dev 21:987-94
Auchampach, John A; Maas, Jason E; Wan, Tina C et al. (2011) Are we putting too much stock in mice? J Mol Cell Cardiol 50:584-5
Si-Tayeb, Karim; Noto, Fallon K; Sepac, Ana et al. (2010) Generation of human induced pluripotent stem cells by simple transient transfection of plasmid DNA encoding reprogramming factors. BMC Dev Biol 10:81
Si-Tayeb, Karim; Noto, Fallon K; Nagaoka, Masato et al. (2010) Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells. Hepatology 51:297-305
Van Orman, Jordan R; Weihrauch, Dorothee; Warltier, David C et al. (2009) Myocardial interstitial fluid inhibits proliferation and cardiomyocyte differentiation in pluripotent embryonic stem cells. Am J Physiol Heart Circ Physiol 297:H1369-76