The Histology &Imaging Core will provide a central site and umbrella authority, which together with satellite infrastructure on this campus confers necessary services, instrumentation, supervision and training for PPG members and personnel within their laboratories. The Core's two major functions are (i) to assist characterization of undifferentiated and differentiated phenotypes of mouse and human embryonic stem cells and their derivatives (all Subprojects), and (2) to assist with the unambiguous histologic characterization of engrafted and regenerated myocardium following stem cell transplantation (Subprojects 1, 3, 4). To achieve these goals the core will assume a training and advisory role while providing services including fixation, embedding and sectioning;routine, histochemical and immunohistochemical staining;widefield and fluorescence microscopy;laser-scanning confocal and multi-photon microscopy;electron microscopy; microangiography. By maintaining an organized online database, processing of images to reconstruct and qualitatively analyze the extent of stem cell engraftment in transplanted myocardium will be provided.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL089471-05
Application #
8475647
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
5
Fiscal Year
2013
Total Cost
$127,975
Indirect Cost
$27,387
Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
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