The goal ofthe Pathology Core is to provide professional services and expertise to all Investigators ofthe Program Project Grant. To facilitate these goals the Pathology Core will provide 1) technical assistance for labor intensive histotechnology techniques from tissue processing to specialized staining, 2) professional veterinary pathology support for gross, microscopic and anatomic phenotyping of the CF pig studies, 3) economic benefits through centralization of equipment, experienced professional staff and availability of specialized reagents, and 4) consultation and instruction in specialized morphologic techniques and image analysis. Program Investigators will have full access to the Core's expertise and resources. The Pathology Core will provide its services to Program Investigators in a timely and efficient manner for optimal assessment of tissues.
The Specific Aims of the Pathology Core are: 1) Provide scientific support, expertise and technical labor for microscopic slide processing from tissues, routine to specialized application of histochemical stains, and immunohistochemistry techniques. 2) Provide expertise and resources to develop new morphologic or morphometric techniques as required to facilitate the needs of Project Investigators. 3) Provide veterinary pathology support (though boarded veterinary pathologists) for examination and interpretation of gross to microscopic tissues from the CF pig model and for consultation in experimental design. ? 4) Provide expertise and a uniform framework for the standardized assessment of tissues collected by Project Investigators to define a comprehensive anatomic assessment/phenotype of porcine CF models and experiments.
|Weldon, Sinéad; McNally, Paul; McAuley, Danny F et al. (2014) miR-31 dysregulation in cystic fibrosis airways contributes to increased pulmonary cathepsin S production. Am J Respir Crit Care Med 190:165-74|
|Derscheid, Rachel J; van Geelen, Albert; Berkebile, Abigail R et al. (2014) Increased concentration of iodide in airway secretions is associated with reduced respiratory syncytial virus disease severity. Am J Respir Cell Mol Biol 50:389-97|
|Hoegger, Mark J; Awadalla, Maged; Namati, Eman et al. (2014) Assessing mucociliary transport of single particles in vivo shows variable speed and preference for the ventral trachea in newborn pigs. Proc Natl Acad Sci U S A 111:2355-60|
|Berkebile, Abigail R; McCray Jr, Paul B (2014) Effects of airway surface liquid pH on host defense in cystic fibrosis. Int J Biochem Cell Biol 52:124-9|
|Awadalla, Maged; Miyawaki, Shinjiro; Abou Alaiwa, Mahmoud H et al. (2014) Early airway structural changes in cystic fibrosis pigs as a determinant of particle distribution and deposition. Ann Biomed Eng 42:915-27|
|Reznikov, Leah R; Abou Alaiwa, Mahmoud H; Dohrn, Cassie L et al. (2014) Antibacterial properties of the CFTR potentiator ivacaftor. J Cyst Fibros 13:515-9|
|Hoegger, Mark J; Fischer, Anthony J; McMenimen, James D et al. (2014) Impaired mucus detachment disrupts mucociliary transport in a piglet model of cystic fibrosis. Science 345:818-22|
|Sun, Xingshen; Olivier, Alicia K; Liang, Bo et al. (2014) Lung phenotype of juvenile and adult cystic fibrosis transmembrane conductance regulator-knockout ferrets. Am J Respir Cell Mol Biol 50:502-12|
|Gibson-Corley, K N; Olivier, A K; Meyerholz, D K (2013) Principles for Valid Histopathologic Scoring in Research. Vet Pathol :|
|Ramachandran, Shyam; Karp, Philip H; Osterhaus, Samantha R et al. (2013) Post-transcriptional regulation of cystic fibrosis transmembrane conductance regulator expression and function by microRNAs. Am J Respir Cell Mol Biol 49:544-51|
Showing the most recent 10 out of 38 publications