The purpose of Core C is to provide state-of-the-art measurements of reactive oxygen species (ROS), reduced glutathione (GSH), glutathione disulfide (GSSG), oxidative modifications of cysteine residues of redox-sensitive proteins in cells, tissues and membranes for participants of the PPG. Core C will assist with preparation and processing of various biological samples for analysis using Electron Spin Resonance (ESR) spectroscopy, High Perfonnance Liquid Chromatography (HPLC) and nano-LC MS/MS analysis. This Core facility has existed since 1999 and has supported numerous studies of superoxide (O2*), hydrogen peroxide (H2O2) and NO in vascular cells and tissue, and it was an essential part of the previous program project studies by Drs. Harrison, Griendling, Taylor and Jo (1-10). This facility will significantly enhance the work of the individual laboratories. and will enhance collaborations between participants of the PPG

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL095070-04
Application #
8380238
Study Section
Special Emphasis Panel (ZHL1-PPG-A)
Project Start
Project End
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
4
Fiscal Year
2012
Total Cost
$170,684
Indirect Cost
$60,640
Name
Emory University
Department
Type
DUNS #
066469933
City
Atlanta
State
GA
Country
United States
Zip Code
30322
Lee, Minyoung; San Martín, Alejandra; Valdivia, Alejandra et al. (2016) Redox-Sensitive Regulation of Myocardin-Related Transcription Factor (MRTF-A) Phosphorylation via Palladin in Vascular Smooth Muscle Cell Differentiation Marker Gene Expression. PLoS One 11:e0153199
Simmons, Rachel D; Kumar, Sandeep; Thabet, Salim Raid et al. (2016) Omics-based approaches to understand mechanosensitive endothelial biology and atherosclerosis. Wiley Interdiscip Rev Syst Biol Med 8:378-401
Montaniel, Kim Ramil C; Harrison, David G (2016) Is Hypertension a Bone Marrow Disease? Circulation 134:1369-1372
Hansen, Laura M; Gupta, Divya; Joseph, Giji et al. (2016) The receptor for advanced glycation end products impairs collateral formation in both diabetic and non-diabetic mice. Lab Invest :
Simmons, Rachel D; Kumar, Sandeep; Jo, Hanjoong (2016) The role of endothelial mechanosensitive genes in atherosclerosis and omics approaches. Arch Biochem Biophys 591:111-31
Griendling, Kathy K; Touyz, Rhian M; Zweier, Jay L et al. (2016) Measurement of Reactive Oxygen Species, Reactive Nitrogen Species, and Redox-Dependent Signaling in the Cardiovascular System: A Scientific Statement From the American Heart Association. Circ Res 119:e39-75
Paredes, Felipe; Parra, Valentina; Torrealba, Natalia et al. (2016) HERPUD1 protects against oxidative stress-induced apoptosis through downregulation of the inositol 1,4,5-trisphosphate receptor. Free Radic Biol Med 90:206-18
Fernández Esmerats, Joan; Heath, Jack; Jo, Hanjoong (2016) Shear-Sensitive Genes in Aortic Valve Endothelium. Antioxid Redox Signal 25:401-14
Di Marco, Elyse; Gray, Stephen P; Kennedy, Kit et al. (2016) NOX4-derived reactive oxygen species limit fibrosis and inhibit proliferation of vascular smooth muscle cells in diabetic atherosclerosis. Free Radic Biol Med 97:556-67
Rathan, Swetha; Ankeny, Casey J; Arjunon, Sivakkumar et al. (2016) Identification of side- and shear-dependent microRNAs regulating porcine aortic valve pathogenesis. Sci Rep 6:25397

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