Research in our laboratory in the last several years has shown that T cells are essential for the development of hypertension. We have found that RAG-1"'" mice, which lack both T and B lymphocytes, develop very blunted hypertensive responses to either chronic low-dose angiotensin II infusion or to DOCA-salt challenge. Adoptive transfer of T, but not B, lymphocytes completely restores the pressor responses to these stimuli. We have found that angiotensin II stimulates a modest increase in T cell expression of the tissue homing markers CCR5 and CD44, which are characteristic of effector T cells. We believe this is important for the genesis of hypertension because we find a striking increase in infiltration of T cells into the perivascular fat around both the aorta and mesenteric vessels in hypertensive mice. The overall hypothesis to be tested in this project is that T cell activation via diverse mechanisms markedly augments the hypertensive response to challenges that normally have no or only minimal effect on blood pressure and that therapy with immunomodulatory agents is a promising approach for treatment of hypertension.
In aim 1, we will test this hypothesis by immunizing mice with antigen from the dental pathogen Porphyromonas gingivalis (P. gingivalis) and then present hypertensive stimuli including angiotensin II, DOCA-salt hypertension and a central stimulus for hypertension. Stimuli that promote THi and TH2 cell polarization will be employed. Preliminary data indicate that previous immunization of mice leads to severe hypertension upon challenge with a low dose of angiotensin II that normally causes minimal increase in blood pressure. An important proinflammatory cytokine, independent of TH1/TH2 cytokines, is IL-17, which we find to be present in the endothelium and perivascular tissues of angiotensin IItreated mice. Our preliminary data in IL17''" mice indicate that it contributes to angiotensin ll-induced hypertension. We will examine the role of IL-17 in the response to angiotensin II and DOCA salt hypertension in naive mice and in mice after immunological stimulation with P. gingivalis lysate by studying IL-17 deficient mice. In preliminary data, we have found that angiotensin II stimulates an increase in renal medullary superoxide levels and a concomitant decrease in renal nitric oxide, and that this is absent in mice lacking T and B cells. We also find that RAG1'''mice exhibit a striking diuresis and natriuresis when challenged with angiotensin II.
In aim 3, we will examine the role of T cells on these renal parameters during angiotensin llinduced hypertension and determine if pre-immunization enhances these effects. Finally in aim 4, we will test the potential for two therapies that can affect either T cell activation or tissue homing to prevent hypertension. In summary, these studies will provide new information regarding the inflammatory processes underlying hypertension and promise to provide new treatment strategies for this common disease
|Lee, Minyoung; San MartÃn, Alejandra; Valdivia, Alejandra et al. (2016) Redox-Sensitive Regulation of Myocardin-Related Transcription Factor (MRTF-A) Phosphorylation via Palladin in Vascular Smooth Muscle Cell Differentiation Marker Gene Expression. PLoS One 11:e0153199|
|Simmons, Rachel D; Kumar, Sandeep; Thabet, Salim Raid et al. (2016) Omics-based approaches to understand mechanosensitive endothelial biology and atherosclerosis. Wiley Interdiscip Rev Syst Biol Med 8:378-401|
|Montaniel, Kim Ramil C; Harrison, David G (2016) Is Hypertension a Bone Marrow Disease? Circulation 134:1369-1372|
|Hansen, Laura M; Gupta, Divya; Joseph, Giji et al. (2016) The receptor for advanced glycation end products impairs collateral formation in both diabetic and non-diabetic mice. Lab Invest :|
|Simmons, Rachel D; Kumar, Sandeep; Jo, Hanjoong (2016) The role of endothelial mechanosensitive genes in atherosclerosis and omics approaches. Arch Biochem Biophys 591:111-31|
|Griendling, Kathy K; Touyz, Rhian M; Zweier, Jay L et al. (2016) Measurement of Reactive Oxygen Species, Reactive Nitrogen Species, and Redox-Dependent Signaling in the Cardiovascular System: A Scientific Statement From the American Heart Association. Circ Res 119:e39-75|
|Paredes, Felipe; Parra, Valentina; Torrealba, Natalia et al. (2016) HERPUD1 protects against oxidative stress-induced apoptosis through downregulation of the inositol 1,4,5-trisphosphate receptor. Free Radic Biol Med 90:206-18|
|FernÃ¡ndez Esmerats, Joan; Heath, Jack; Jo, Hanjoong (2016) Shear-Sensitive Genes in Aortic Valve Endothelium. Antioxid Redox Signal 25:401-14|
|Di Marco, Elyse; Gray, Stephen P; Kennedy, Kit et al. (2016) NOX4-derived reactive oxygen species limit fibrosis and inhibit proliferation of vascular smooth muscle cells in diabetic atherosclerosis. Free Radic Biol Med 97:556-67|
|Rathan, Swetha; Ankeny, Casey J; Arjunon, Sivakkumar et al. (2016) Identification of side- and shear-dependent microRNAs regulating porcine aortic valve pathogenesis. Sci Rep 6:25397|
Showing the most recent 10 out of 91 publications