The purpose of Core C is to provide state-of-the-art measurements of reactive oxygen species (ROS), reduced glutathione (GSH), glutathione disulfide (GSSG), oxidative modifications of cysteine residues of redox-sensitive proteins in cells, tissues and membranes for participants of the PPG. Core C will assist with preparation and processing of various biological samples for analysis using Electron Spin Resonance (ESR) spectroscopy, High Perfonnance Liquid Chromatography (HPLC) and nano-LC MS/MS analysis. This Core facility has existed since 1999 and has supported numerous studies of superoxide (O2*), hydrogen peroxide (H2O2) and NO in vascular cells and tissue, and it was an essential part of the previous program project studies by Drs. Harrison, Griendling, Taylor and Jo (1-10). This facility will significantly enhance the work of the individual laboratories. and will enhance collaborations between participants of the PPG

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Special Emphasis Panel (ZHL1-PPG-A)
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Emory University
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