Core C consists of the director as well as one technician who will provide molecular and cellular biology support for each of the three projects in this program project proposal. This core will provide three major functions which will be to assist each ofthe laboratories in experiments designed to;1) manipulate gene expression within vascular smooth muscle cells in vitro and in vivo;2) produce and manipulate recombinant proteins to be applied to vessels or cells;and 3) to isolate ceils from wild-type or knockout mutant animals and to culture them in two-dimensional or three-dimensional extracellular environments. The core director has considerable experience in the creation of recombinant viral vectors for gene delivery into vascular cells as well as siRNA technology to suppress gene expression in primary vascular cells including vascular smooth muscle cells, pericytes and endothelial cells. In addition, the core director has extensive experience in the culture and manipulation of primary vascular cells in either twodimensional or three-dimensional culture. This core will also provide an important function to facilitate each investigator to advance gene expression technologies within microvessels to be able to manipulate microcirculatory function. The director will participate in all scientific functions related to the proposal to be able to properly serve the needs of each project and to facilitate the molecular aspects of each research direction. This core will provide services that are essential for the overall function and scientific integration of the Program Project Grant. All projects will rely on the services provided by this core in each of the three main areas of focus described above.

Public Health Relevance

This core will provide services that are essential for the overall function and scientific integration of the Program Project Grant. All projects will rely on the services provided by this core in each ofthe three main areas of focus described above.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL095486-04
Application #
8462672
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-03-01
Budget End
2014-02-28
Support Year
4
Fiscal Year
2013
Total Cost
$106,869
Indirect Cost
$36,386
Name
University of Missouri-Columbia
Department
Type
DUNS #
153890272
City
Columbia
State
MO
Country
United States
Zip Code
65211
Manrique, Camila; Habibi, Javad; Aroor, Annayya R et al. (2016) Dipeptidyl peptidase-4 inhibition with linagliptin prevents western diet-induced vascular abnormalities in female mice. Cardiovasc Diabetol 15:94
Foote, Christopher A; Castorena-Gonzalez, Jorge A; Staiculescu, Marius C et al. (2016) Brief serotonin exposure initiates arteriolar inward remodeling processes in vivo that involve transglutaminase activation and actin cytoskeleton reorganization. Am J Physiol Heart Circ Physiol 310:H188-98
Wang, Derek Z; Jones, Allan W; Wang, Walter Z et al. (2016) Soluble guanylate cyclase activation during ischemic injury in mice protects against postischemic inflammation at the mitochondrial level. Am J Physiol Gastrointest Liver Physiol 310:G747-56
Higashi, Yusuke; Sukhanov, Sergiy; Shai, Shaw-Yung et al. (2016) Insulin-Like Growth Factor-1 Receptor Deficiency in Macrophages Accelerates Atherosclerosis and Induces an Unstable Plaque Phenotype in Apolipoprotein E-Deficient Mice. Circulation 133:2263-78
Sehgel, Nancy L; Sun, Zhe; Hong, Zhongkui et al. (2015) Augmented vascular smooth muscle cell stiffness and adhesion when hypertension is superimposed on aging. Hypertension 65:370-7
Scallan, Joshua P; Hill, Michael A; Davis, Michael J (2015) Lymphatic vascular integrity is disrupted in type 2 diabetes due to impaired nitric oxide signalling. Cardiovasc Res 107:89-97
Dudenbostel, Tanja; Acelajado, Maria C; Pisoni, Roberto et al. (2015) Refractory Hypertension: Evidence of Heightened Sympathetic Activity as a Cause of Antihypertensive Treatment Failure. Hypertension 66:126-33
Nance, Michael E; Whitfield, Justin T; Zhu, Yi et al. (2015) Attenuated sarcomere lengthening of the aged murine left ventricle observed using two-photon fluorescence microscopy. Am J Physiol Heart Circ Physiol 309:H918-25
Fairfax, Seth T; Padilla, Jaume; Vianna, Lauro C et al. (2015) Myogenic responses occur on a beat-to-beat basis in the resting human limb. Am J Physiol Heart Circ Physiol 308:H59-67
Zuidema, Mozow Y; Korthuis, Ronald J (2015) Intravital microscopic methods to evaluate anti-inflammatory effects and signaling mechanisms evoked by hydrogen sulfide. Methods Enzymol 555:93-125

Showing the most recent 10 out of 48 publications