Core C consists of the director as well as one technician who will provide molecular and cellular biology support for each of the three projects in this program project proposal. This core will provide three major functions which will be to assist each ofthe laboratories in experiments designed to;1) manipulate gene expression within vascular smooth muscle cells in vitro and in vivo;2) produce and manipulate recombinant proteins to be applied to vessels or cells;and 3) to isolate ceils from wild-type or knockout mutant animals and to culture them in two-dimensional or three-dimensional extracellular environments. The core director has considerable experience in the creation of recombinant viral vectors for gene delivery into vascular cells as well as siRNA technology to suppress gene expression in primary vascular cells including vascular smooth muscle cells, pericytes and endothelial cells. In addition, the core director has extensive experience in the culture and manipulation of primary vascular cells in either twodimensional or three-dimensional culture. This core will also provide an important function to facilitate each investigator to advance gene expression technologies within microvessels to be able to manipulate microcirculatory function. The director will participate in all scientific functions related to the proposal to be able to properly serve the needs of each project and to facilitate the molecular aspects of each research direction. This core will provide services that are essential for the overall function and scientific integration of the Program Project Grant. All projects will rely on the services provided by this core in each of the three main areas of focus described above.

Public Health Relevance

This core will provide services that are essential for the overall function and scientific integration of the Program Project Grant. All projects will rely on the services provided by this core in each ofthe three main areas of focus described above.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL095486-04
Application #
8462672
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-03-01
Budget End
2014-02-28
Support Year
4
Fiscal Year
2013
Total Cost
$106,869
Indirect Cost
$36,386
Name
University of Missouri-Columbia
Department
Type
DUNS #
153890272
City
Columbia
State
MO
Country
United States
Zip Code
65211
Sehgel, Nancy L; Sun, Zhe; Hong, Zhongkui et al. (2015) Augmented vascular smooth muscle cell stiffness and adhesion when hypertension is superimposed on aging. Hypertension 65:370-7
Hong, Zhongkui; Sun, Zhe; Li, Min et al. (2014) Vasoactive agonists exert dynamic and coordinated effects on vascular smooth muscle cell elasticity, cytoskeletal remodelling and adhesion. J Physiol 592:1249-66
Kalogeris, Theodore; Bao, Yimin; Korthuis, Ronald J (2014) Mitochondrial reactive oxygen species: a double edged sword in ischemia/reperfusion vs preconditioning. Redox Biol 2:702-14
Sun, Zhe; Parrish, Alan R; Hill, Michael A et al. (2014) N-cadherin, a vascular smooth muscle cell-cell adhesion molecule: function and signaling for vasomotor control. Microcirculation 21:208-18
Kalogeris, Theodore J; Baines, Christopher; Korthuis, Ronald J (2014) Adenosine prevents TNF?-induced decrease in endothelial mitochondrial mass via activation of eNOS-PGC-1? regulatory axis. PLoS One 9:e98459
Nourian, Zahra; Li, Min; Leo, M Dennis et al. (2014) Large conductance Ca2+-activated K+ channel (BKCa) ?-subunit splice variants in resistance arteries from rat cerebral and skeletal muscle vasculature. PLoS One 9:e98863
Fairfax, Seth T; Holwerda, Seth W; Credeur, Daniel P et al. (2013) The role of ýý-adrenergic receptors in mediating beat-by-beat sympathetic vascular transduction in the forearm of resting man. J Physiol 591:3637-49
Korthuis, Ronald J; Kalogeris, Theodore (2013) TRPing up reperfusion: neutrophil TRPM2 channels exacerbate necrosis and contractile dysfunction in post-ischaemic myocardium. Cardiovasc Res 97:197-9
Hong, Zhongkui; Ersoy, Ilker; Sun, Mingzhai et al. (2013) Influence of membrane cholesterol and substrate elasticity on endothelial cell spreading behavior. J Biomed Mater Res A 101:1994-2004
Fairfax, Seth T; Padilla, Jaume; Vianna, Lauro C et al. (2013) Spontaneous bursts of muscle sympathetic nerve activity decrease leg vascular conductance in resting humans. Am J Physiol Heart Circ Physiol 304:H759-66

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