Imaging of intracellular calcium signaling is a key approach used by each Investigator on this PPG. This PPG employs.extensive imaging of a variety of cell types (smooth muscle, endothelium, astrocytes), at the level of isolated single cells (endothelial and smooth muscle cells) and multicellular preparations (isolated arteries, brain slices), each of them exhibiting calcium signals with different spatio-temporal dynamics (Ca^* pulsars, Ca^"" sparks, Ca^"" waves, global Ca^* changes). In addition, some studies require Ca^^ imaging combined with simultaneous electrophysiological and arterial diameter measurements. To address the complexity of imaging different calcium signals/tissues we use a variety of synthetic (fura-2, fluo-4) and genetically encoded (GCaMP2, GCaMP2-mCherry) calcium indicators. To adequately image such a diverse assortment of tissues requires the use of specialized imaging instruments with different features. The Imaging Core provides access for each investigator to expensive (over $1,000,000), state-of-the-art equipment that will address the needs of each proposal. By consolidating the imaging equipment into a single Core we will reduce expenses for collecting imaging data. The Core equipment consists of four wide-field epi-fluorescent systems, three laser scanning confocal microscopes and one multiphoton imaging system. Each setup is housed in a separate dark room in the connected Given and HSRF buildings of the University of Vermont. The specific objectives of the Imaging Core are to maintain the instruments in optimal condition, coordinate scheduling to maximize the use of each instrument, train users unfamiliar with software or instrumentation, provide expertise to investigators in experimental design, processing, analysis and interpretation of the experimental data, and create and maintain custom software for data analysis.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL095488-05
Application #
8722009
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
5
Fiscal Year
2014
Total Cost
$217,967
Indirect Cost
$73,237
Name
University of Vermont & St Agric College
Department
Type
DUNS #
066811191
City
Burlington
State
VT
Country
United States
Zip Code
05405
Wallace, Kedra; Tremble, Sarah M; Owens, Michelle Y et al. (2015) Plasma from patients with HELLP syndrome increases blood-brain barrier permeability. Reprod Sci 22:278-84
Cipolla, Marilyn J; Sweet, Julie; Chan, Siu-Lung et al. (2014) Increased pressure-induced tone in rat parenchymal arterioles vs. middle cerebral arteries: role of ion channels and calcium sensitivity. J Appl Physiol (1985) 117:53-9
Longden, Thomas A; Dabertrand, Fabrice; Hill-Eubanks, David C et al. (2014) Stress-induced glucocorticoid signaling remodels neurovascular coupling through impairment of cerebrovascular inwardly rectifying K+ channel function. Proc Natl Acad Sci U S A 111:7462-7
Dunn, Kathryn M; Nelson, Mark T (2014) Neurovascular signaling in the brain and the pathological consequences of hypertension. Am J Physiol Heart Circ Physiol 306:H1-14
Cipolla, Marilyn J; Chan, Siu-Lung; Sweet, Julie et al. (2014) Postischemic reperfusion causes smooth muscle calcium sensitization and vasoconstriction of parenchymal arterioles. Stroke 45:2425-30
Mingin, Gerald C; Peterson, Abbey; Erickson, Cuixia Shi et al. (2014) Social stress induces changes in urinary bladder function, bladder NGF content, and generalized bladder inflammation in mice. Am J Physiol Regul Integr Comp Physiol 307:R893-900
Schreurs, Malou P H; Cipolla, Marilyn J (2014) Cerebrovascular dysfunction and blood-brain barrier permeability induced by oxidized LDL are prevented by apocynin and magnesium sulfate in female rats. J Cardiovasc Pharmacol 63:33-9
Krishnamoorthy, Gayathri; Sonkusare, Swapnil K; Heppner, Thomas J et al. (2014) Opposing roles of smooth muscle BK channels and ryanodine receptors in the regulation of nerve-evoked constriction of mesenteric resistance arteries. Am J Physiol Heart Circ Physiol 306:H981-8
Mercado, Jose; Baylie, Rachael; Navedo, Manuel F et al. (2014) Local control of TRPV4 channels by AKAP150-targeted PKC in arterial smooth muscle. J Gen Physiol 143:559-75
Hill-Eubanks, David C; Gonzales, Albert L; Sonkusare, Swapnil K et al. (2014) Vascular TRP channels: performing under pressure and going with the flow. Physiology (Bethesda) 29:343-60

Showing the most recent 10 out of 42 publications