The Electrophysiology Core will provide centralized services for electrophysiological studies, including preparation of in vitro brain slices, patch-clamp recordings, immunohistochemistry, and the associated image and data analysis. Project 3 (Scammell) and Project 5 (Chamberlin) will use this Core facility. Project 3 will use the services of the Core to examine how orexin and dynorphin increase forebrain activity. Specifically, these experiments will define the pre- and post-synaptic effects and the mechanism through which these neurotransmitters affect cortically-projecting neurons in the basal forebrain. We will also determine the peptide receptors and conductances involved in these responses. Project 5 will use the Electrophysiology Core to determine whether neurons of the Kolliker-Fuse (KF) nucleus are chemosensitive. We will determine which KF neurons are intrinsically responsive to changes of pH, and we will identify the mechanisms underlying these responses. These Projects will benefit from the expertise and many shared resources of the Core.
The goal of the Electrophysiology Core is to provide resources and expertise for in vitro recordings for multiple investigators. The Projects will benefit from using a well-established in vitro electrophysiology laboratory. This centralized service will ensure consistent, high-quality recordings across Projects and will prevent duplication of equipment and resources.
|Yokota, Shigefumi; Kaur, Satvinder; VanderHorst, Veronique G et al. (2015) Respiratory-related outputs of glutamatergic, hypercapnia-responsive parabrachial neurons in mice. J Comp Neurol 523:907-20|
|Eckert, Danny J; Malhotra, Atul; Wellman, Andrew et al. (2014) Trazodone increases the respiratory arousal threshold in patients with obstructive sleep apnea and a low arousal threshold. Sleep 37:811-9|
|Pittman-Polletta, Benjamin; Hsieh, Wan-Hsin; Kaur, Satvinder et al. (2014) Detecting phase-amplitude coupling with high frequency resolution using adaptive decompositions. J Neurosci Methods 226:15-32|
|Sands, Scott A; Eckert, Danny J; Jordan, Amy S et al. (2014) Enhanced upper-airway muscle responsiveness is a distinct feature of overweight/obese individuals without sleep apnea. Am J Respir Crit Care Med 190:930-7|
|Shah, Ravi V; Abbasi, Siddique A; Heydari, Bobak et al. (2014) Obesity and sleep apnea are independently associated with adverse left ventricular remodeling and clinical outcome in patients with atrial fibrillation and preserved ventricular function. Am Heart J 167:620-6|
|Bakker, Jessie P; Edwards, Bradley A; Gautam, Shiva P et al. (2014) Blood pressure improvement with continuous positive airway pressure is independent of obstructive sleep apnea severity. J Clin Sleep Med 10:365-9|
|Yang, Chun; McKenna, James T; Zant, Janneke C et al. (2014) Cholinergic neurons excite cortically projecting basal forebrain GABAergic neurons. J Neurosci 34:2832-44|
|McSharry, David G; Saboisky, Julian P; Deyoung, Pam et al. (2014) Physiological mechanisms of upper airway hypotonia during REM sleep. Sleep 37:561-9|
|Zielinski, Mark R; Kim, Youngsoo; Karpova, Svetlana A et al. (2014) Chronic sleep restriction elevates brain interleukin-1 beta and tumor necrosis factor-alpha and attenuates brain-derived neurotrophic factor expression. Neurosci Lett 580:27-31|
|Weng, F J; Williams, R H; Hawryluk, J M et al. (2014) Carbachol excites sublaterodorsal nucleus neurons projecting to the spinal cord. J Physiol 592:1601-17|
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