The Electrophysiology Core will provide centralized services for electrophysiological studies, including preparation of in vitro brain slices, patch-clamp recordings, immunohistochemistry, and the associated image and data analysis. Project 3 (Scammell) and Project 5 (Chamberlin) will use this Core facility. Project 3 will use the services of the Core to examine how orexin and dynorphin increase forebrain activity. Specifically, these experiments will define the pre- and post-synaptic effects and the mechanism through which these neurotransmitters affect cortically-projecting neurons in the basal forebrain. We will also determine the peptide receptors and conductances involved in these responses. Project 5 will use the Electrophysiology Core to determine whether neurons of the Kolliker-Fuse (KF) nucleus are chemosensitive. We will determine which KF neurons are intrinsically responsive to changes of pH, and we will identify the mechanisms underlying these responses. These Projects will benefit from the expertise and many shared resources of the Core.

Public Health Relevance

The goal of the Electrophysiology Core is to provide resources and expertise for in vitro recordings for multiple investigators. The Projects will benefit from using a well-established in vitro electrophysiology laboratory. This centralized service will ensure consistent, high-quality recordings across Projects and will prevent duplication of equipment and resources.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL095491-04
Application #
8435433
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-03-01
Budget End
2014-02-28
Support Year
4
Fiscal Year
2013
Total Cost
$178,233
Indirect Cost
$92,950
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02215
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