The Neuroanatomv-lmaging Core (Core C) is an integral part of the Program, providing resources and support for structural analysis as required in each of the proposed studies. Core personnel include a Director, a Co- Director, a Technical Director, and a technician. The Director, Dr. Milner, has an internationally recognized reputation in the areas of light (LM) and electron (EM) microscopy, and an extensive number of publications in these areas (see publications (P) for Project 3). The Co-Director, Dr. Pierce, is an experienced quantitative anatomist, having conducted studies involving correlated EM and LM stereological analysis and reconstruction (see Biosketch). The facilities and services provided by this core will support;(a) the selective labeling of neuronal subgroups through tract-tracer injection methodologies;(b) the fixation and processing of rodent tissue for LM, confocal and EM analysis, (c) single and dual preembedding and postembedding immunolabeling of antigens, and antibody characterization, (d) in situ hybridization, (e) reactive oxygen species (ROS) and nitric oxide (NO) imaging and (f) image acquisition for both analysis and presentation. Analytic approaches supported by the Neuroanatomv-lmaging Core will include: plotting neuronal distributions, the stereological determination of neuronal number, EM serial analysis and reconstruction of portions of labeled neurons and their processes, fluorescent densitometry, and the quantification of EM immunolabeling patterns (such as determining the extent to which processes are double-labeled, or immunogold particles are partitioned between different types of membranes, or membranes and cytoplasmic compartments).

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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Weill Medical College of Cornell University
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Van Kempen, Tracey A; Gorecka, Jolanta; Gonzalez, Andreina D et al. (2014) Characterization of neural estrogen signaling and neurotrophic changes in the accelerated ovarian failure mouse model of menopause. Endocrinology 155:3610-23
Marques-Lopes, Jose; Van Kempen, Tracey; Waters, Elizabeth M et al. (2014) Slow-pressor angiotensin II hypertension and concomitant dendritic NMDA receptor trafficking in estrogen receptor ?-containing neurons of the mouse hypothalamic paraventricular nucleus are sex and age dependent. J Comp Neurol 522:3075-90
Jackman, Katherine A; Zhou, Ping; Faraco, Giuseppe et al. (2014) Dichotomous effects of chronic intermittent hypoxia on focal cerebral ischemic injury. Stroke 45:1460-7
Almey, Anne; Cannell, Elizabeth; Bertram, Kyla et al. (2014) Medial prefrontal cortical estradiol rapidly alters memory system bias in female rats: ultrastructural analysis reveals membrane-associated estrogen receptors as potential mediators. Endocrinology 155:4422-32
Faraco, Giuseppe; Wijasa, Teodora Stella; Park, Laibaik et al. (2014) Water deprivation induces neurovascular and cognitive dysfunction through vasopressin-induced oxidative stress. J Cereb Blood Flow Metab 34:852-60
Iadecola, Costantino (2014) Hypertension and dementia. Hypertension 64:3-5
Pierce, Joseph P; Kelter, David T; McEwen, Bruce S et al. (2014) Hippocampal mossy fiber leu-enkephalin immunoreactivity in female rats is significantly altered following both acute and chronic stress. J Chem Neuroanat 55:9-17
Milner, Teresa A; Burstein, Suzanne R; Marrone, Gina F et al. (2013) Stress differentially alters mu opioid receptor density and trafficking in parvalbumin-containing interneurons in the female and male rat hippocampus. Synapse 67:757-72
Iadecola, Costantino (2013) The pathobiology of vascular dementia. Neuron 80:844-66
Faraco, Giuseppe; Moraga, Ana; Moore, Jamie et al. (2013) Circulating endothelin-1 alters critical mechanisms regulating cerebral microcirculation. Hypertension 62:759-66

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