The Neuroanatomv-lmaging Core (Core C) is an integral part of the Program, providing resources and support for structural analysis as required in each of the proposed studies. Core personnel include a Director, a Co- Director, a Technical Director, and a technician. The Director, Dr. Milner, has an internationally recognized reputation in the areas of light (LM) and electron (EM) microscopy, and an extensive number of publications in these areas (see publications (P) for Project 3). The Co-Director, Dr. Pierce, is an experienced quantitative anatomist, having conducted studies involving correlated EM and LM stereological analysis and reconstruction (see Biosketch). The facilities and services provided by this core will support;(a) the selective labeling of neuronal subgroups through tract-tracer injection methodologies;(b) the fixation and processing of rodent tissue for LM, confocal and EM analysis, (c) single and dual preembedding and postembedding immunolabeling of antigens, and antibody characterization, (d) in situ hybridization, (e) reactive oxygen species (ROS) and nitric oxide (NO) imaging and (f) image acquisition for both analysis and presentation. Analytic approaches supported by the Neuroanatomv-lmaging Core will include: plotting neuronal distributions, the stereological determination of neuronal number, EM serial analysis and reconstruction of portions of labeled neurons and their processes, fluorescent densitometry, and the quantification of EM immunolabeling patterns (such as determining the extent to which processes are double-labeled, or immunogold particles are partitioned between different types of membranes, or membranes and cytoplasmic compartments).

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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Weill Medical College of Cornell University
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Mende, Michael; Fletcher, Emily V; Belluardo, Josephine L et al. (2016) Sensory-Derived Glutamate Regulates Presynaptic Inhibitory Terminals in Mouse Spinal Cord. Neuron 90:1189-202
Faraco, Giuseppe; Park, Laibaik; Zhou, Ping et al. (2016) Hypertension enhances Aβ-induced neurovascular dysfunction, promotes β-secretase activity, and leads to amyloidogenic processing of APP. J Cereb Blood Flow Metab 36:241-52
Garzón, Miguel; Pickel, Virginia M (2016) Electron microscopic localization of M2-muscarinic receptors in cholinergic and noncholinergic neurons of the laterodorsal tegmental and pedunculopontine nuclei of the rat mesopontine tegmentum. J Comp Neurol 524:3084-103
Almey, Anne; Milner, Teresa A; Brake, Wayne G (2016) Estrogen receptor α and G-protein coupled estrogen receptor 1 are localized to GABAergic neurons in the dorsal striatum. Neurosci Lett 622:118-23
Van Kempen, Tracey A; Narayan, Ankita; Waters, Elizabeth M et al. (2016) Alterations in the subcellular distribution of NADPH oxidase p47(phox) in hypothalamic paraventricular neurons following slow-pressor angiotensin II hypertension in female mice with accelerated ovarian failure. J Comp Neurol 524:2251-65
Mazid, Sanoara; Hall, Baila S; Odell, Shannon C et al. (2016) Sex differences in subcellular distribution of delta opioid receptors in the rat hippocampus in response to acute and chronic stress. Neurobiol Stress 5:37-53
Marques-Lopes, Jose; Lynch, Mary-Katherine; Van Kempen, Tracey A et al. (2015) Female protection from slow-pressor effects of angiotensin II involves prevention of ROS production independent of NMDA receptor trafficking in hypothalamic neurons expressing angiotensin 1A receptors. Synapse 69:148-65
Ma, Qian; Yang, Jianmin; Li, Thomas et al. (2015) Selective reduction of striatal mature BDNF without induction of proBDNF in the zQ175 mouse model of Huntington's disease. Neurobiol Dis 82:466-77
Waters, Elizabeth M; Thompson, Louisa I; Patel, Parth et al. (2015) G-protein-coupled estrogen receptor 1 is anatomically positioned to modulate synaptic plasticity in the mouse hippocampus. J Neurosci 35:2384-97
Van Kempen, T A; Dodos, M; Woods, C et al. (2015) Sex differences in NMDA GluN1 plasticity in rostral ventrolateral medulla neurons containing corticotropin-releasing factor type 1 receptor following slow-pressor angiotensin II hypertension. Neuroscience 307:83-97

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