The purpose of Core B - Mouse Models of Pulmonary Inflammation - is to provide a resource for investigators performing studies in mice. The Director of Core B is Charies W. Frevert, DVM, ScD, who has extensive experience in the use of mouse models of lung inflammation and infection. The Co-lnvestigator is Andrew Burich, DVM, MS who has research experience with mouse models of inflammation. To meet the needs of the program projects we have developed four goals. The first goal is to provide access to the expertise, resources, and facilities required to model pulmonary inflammation in mice. This includes access to individuals with expertise in comparative pathology and pulmonary inflammation;access to BSL-2 and BSL-3 laboratories to carry out studies on lung infection in mice;and access to a Transgenic Animal Resource Laboratory to develop genetically engineered mice proposed by the four projects. The second goal will be to standardize the mouse models of pulmonary inflammation and lung infection used by the different program projects. This is important as it will facilitate interactions between projects. The third goal is to isolate and distribute primary cell cultures obtained from mice. This includes isolation of primary cell cultures of epithelial cells grown at an air liquid interface, lung fibroblasts, bone marrow derived macrophages, and alveolar macrophages. The fourth goal will be to interact on a regular basis with Robert Vernon, PhD the Director of Core C. This interaction is important because it will insure that sample collection and submission from Core B to Core C is standardized. Drs. Frevert and Vernon will also work closely together to insure that all four projects have access to specialized imaging systems and image analysis programs required for their work. By consolidating skilled staff, facilities, and equipment in Core B and making it available to all projects, we will reduce the cost of producing high-quality data and will facilitate interactions among the different projects. Therefore, Core B will play an important role in the successful completion of work proposed in this program project.

Public Health Relevance

Lung infections place a higher burden on public health than other major diseases such as HIV/AIDS, cancer, coronary heart disease, and strokes (WHO data). The goal of Core B is to assist all projects model lung infection in mice. The knowledge gained by completion ofthe studies carried out in this core will hopefully lead to novel therapeutic strategies to treat lung infection.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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Benaroya Research Institute at Virginia Mason
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Wilson, S S; Tocchi, A; Holly, M K et al. (2015) A small intestinal organoid model of non-invasive enteric pathogen-epithelial cell interactions. Mucosal Immunol 8:352-61
Giannandrea, Matthew; Parks, William C (2014) Diverse functions of matrix metalloproteinases during fibrosis. Dis Model Mech 7:193-203
Chang, Mary Y; Tanino, Yoshinori; Vidova, Veronika et al. (2014) A rapid increase in macrophage-derived versican and hyaluronan in infectious lung disease. Matrix Biol 34:1-12
Smigiel, Kate S; Richards, Elizabeth; Srivastava, Shivani et al. (2014) CCR7 provides localized access to IL-2 and defines homeostatically distinct regulatory T cell subsets. J Exp Med 211:121-36
Srivastava, Shivani; Koch, Meghan A; Pepper, Marion et al. (2014) Type I interferons directly inhibit regulatory T cells to allow optimal antiviral T cell responses during acute LCMV infection. J Exp Med 211:961-74
Srivastava, Shivani; Koch, Lisa K; Campbell, Daniel J (2014) IFN?R signaling in effector but not regulatory T cells is required for immune dysregulation during type I IFN-dependent inflammatory disease. J Immunol 193:2733-42
Gharib, Sina A; Johnston, Laura K; Huizar, Isham et al. (2014) MMP28 promotes macrophage polarization toward M2 cells and augments pulmonary fibrosis. J Leukoc Biol 95:9-18
Smigiel, Kate S; Srivastava, Shivani; Stolley, J Michael et al. (2014) Regulatory T-cell homeostasis: steady-state maintenance and modulation during inflammation. Immunol Rev 259:40-59
Chang, Mary Y; Tanino, Yoshinori; Vidova, Veronika et al. (2014) Reprint of: A rapid increase in macrophage-derived versican and hyaluronan in infectious lung disease. Matrix Biol 35:162-73
Kang, Inkyung; Yoon, Dong Won; Braun, Kathleen R et al. (2014) Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor ? (TGF?)-, epidermal growth factor (EGF)-, and nuclear factor ?B (NF?B)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion. J Biol Chem 289:15393-404

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