Core C will utilize the equipment and staff of the existing BRI Histology and Flow Cytometry Cores to provide histological processing, photomicrography, and flow cytometry services for the four Projects described in this proposal. For histology. Core C will provide high-throughput equipment for tissue processing, embedding, sectioning, and staining with conventional dyes, antibodies, and nucleic acid probes. For photomicrography, Core C will provide one confocal microscope and five digital camera-equipped conventional microscopes with complementary imaging capabilities. For flow cytometry, Core C will incorporate a variety of multi-featured cell sorting instruments. With regard to human resources, Core C will have experienced staff who will provide Project personnel with a full range of histological prep, consultation, and instrument training services.

Public Health Relevance

The histology, photomicrography, and flow cytometry services provided by Core C will be essential to all four Projects. Histological processing and photomicrography will be necessary to evaluate the responses of lungs to viral and bacterial infection and to TSLP-induced inflammation according to the following parameters: (1) fibrosis and other pathology, (2) expression of specific extracellular matrix components and matrix metalloproteinases, and (3) lymphocyte/macrophage trafficking. Flow cytometry services will be used extensively by Projects 2 and 4 to sort immune cell populations for phenotypic analyses and for adoptive transfer experiments.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL098067-05
Application #
8701352
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
5
Fiscal Year
2014
Total Cost
$233,590
Indirect Cost
$96,942
Name
Benaroya Research Institute at Virginia Mason
Department
Type
DUNS #
076647908
City
Seattle
State
WA
Country
United States
Zip Code
98101
Han, Hongwei; Ziegler, Steven F (2017) Intradermal administration of IL-33 induces allergic airway inflammation. Sci Rep 7:1706
Kang, Inkyung; Harten, Ingrid A; Chang, Mary Y et al. (2017) Versican Deficiency Significantly Reduces Lung Inflammatory Response Induced by Polyinosine-Polycytidylic Acid Stimulation. J Biol Chem 292:51-63
Wight, Thomas N (2017) Provisional matrix: A role for versican and hyaluronan. Matrix Biol 60-61:38-56
Sheih, A; Parks, W C; Ziegler, S F (2017) GM-CSF produced by the airway epithelium is required for sensitization to cockroach allergen. Mucosal Immunol 10:705-715
Wight, Thomas N; Frevert, Charles W; Debley, Jason S et al. (2017) Interplay of extracellular matrix and leukocytes in lung inflammation. Cell Immunol 312:1-14
Gaucherand, Léa; Falk, Ben A; Evanko, Stephen P et al. (2017) Crosstalk Between T Lymphocytes and Lung Fibroblasts: Generation of a Hyaluronan-Enriched Extracellular Matrix Adhesive for Monocytes. J Cell Biochem 118:2118-2130
Han, H; Roan, F; Johnston, L K et al. (2017) IL-33 promotes gastrointestianl allergy in a TSLP-independent manner. Mucosal Immunol :
Secor, Patrick R; Michaels, Lia A; Smigiel, Kate S et al. (2017) Filamentous Bacteriophage Produced by Pseudomonas aeruginosa Alters the Inflammatory Response and Promotes Noninvasive Infection In Vivo. Infect Immun 85:
Han, Hongwei; Roan, Florence; Ziegler, Steven F (2017) The atopic march: current insights into skin barrier dysfunction and epithelial cell-derived cytokines. Immunol Rev 278:116-130
Kashiwagi, Mariko; Hosoi, Junichi; Lai, Jen-Feng et al. (2017) Direct control of regulatory T cells by keratinocytes. Nat Immunol 18:334-343

Showing the most recent 10 out of 79 publications