Core C will utilize the equipment and staff of the existing BRI Histology and Flow Cytometry Cores to provide histological processing, photomicrography, and flow cytometry services for the four Projects described in this proposal. For histology. Core C will provide high-throughput equipment for tissue processing, embedding, sectioning, and staining with conventional dyes, antibodies, and nucleic acid probes. For photomicrography, Core C will provide one confocal microscope and five digital camera-equipped conventional microscopes with complementary imaging capabilities. For flow cytometry, Core C will incorporate a variety of multi-featured cell sorting instruments. With regard to human resources, Core C will have experienced staff who will provide Project personnel with a full range of histological prep, consultation, and instrument training services.

Public Health Relevance

The histology, photomicrography, and flow cytometry services provided by Core C will be essential to all four Projects. Histological processing and photomicrography will be necessary to evaluate the responses of lungs to viral and bacterial infection and to TSLP-induced inflammation according to the following parameters: (1) fibrosis and other pathology, (2) expression of specific extracellular matrix components and matrix metalloproteinases, and (3) lymphocyte/macrophage trafficking. Flow cytometry services will be used extensively by Projects 2 and 4 to sort immune cell populations for phenotypic analyses and for adoptive transfer experiments.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL098067-05
Application #
8701352
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
5
Fiscal Year
2014
Total Cost
$233,590
Indirect Cost
$96,942
Name
Benaroya Research Institute at Virginia Mason
Department
Type
DUNS #
076647908
City
Seattle
State
WA
Country
United States
Zip Code
98101
McMahan, Ryan S; Birkland, Timothy P; Smigiel, Kate S et al. (2016) Stromelysin-2 (MMP10) Moderates Inflammation by Controlling Macrophage Activation. J Immunol 197:899-909
Valladao, Andrea C; Frevert, Charles W; Koch, Lisa K et al. (2016) STAT6 Regulates the Development of Eosinophilic versus Neutrophilic Asthma in Response to Alternaria alternata. J Immunol 197:4541-4551
Stolley, J Michael; Campbell, Daniel J (2016) A 33D1+ Dendritic Cell/Autoreactive CD4+ T Cell Circuit Maintains IL-2-Dependent Regulatory T Cells in the Spleen. J Immunol 197:2635-45
Reeves, Stephen R; Kaber, Gernot; Sheih, Alyssa et al. (2016) Subepithelial Accumulation of Versican in a Cockroach Antigen-Induced Murine Model of Allergic Asthma. J Histochem Cytochem 64:364-80
Merrilees, Mervyn J; Zuo, Ning; Evanko, Stephen P et al. (2016) G1 Domain of Versican Regulates Hyaluronan Organization and the Phenotype of Cultured Human Dermal Fibroblasts. J Histochem Cytochem 64:353-63
Wight, Thomas N (2016) Provisional matrix: A role for versican and hyaluronan. Matrix Biol :
Keire, Paul A; Bressler, Steven L; Mulvihill, Eileen R et al. (2016) Inhibition of versican expression by siRNA facilitates tropoelastin synthesis and elastic fiber formation by human SK-LMS-1 leiomyosarcoma smooth muscle cells in vitro and in vivo. Matrix Biol 50:67-81
Vandivort, Tyler C; An, Dowon; Parks, William C (2016) An Improved Method for Rapid Intubation of the Trachea in Mice. J Vis Exp :53771
Piliponsky, Adrian M; Lahiri, Asha; Truong, Phuong et al. (2016) Thymic Stromal Lymphopoietin Improves Survival and Reduces Inflammation in Sepsis. Am J Respir Cell Mol Biol 55:264-74
Sheih, A; Parks, W C; Ziegler, S F (2016) GM-CSF produced by the airway epithelium is required for sensitization to cockroach allergen. Mucosal Immunol :

Showing the most recent 10 out of 71 publications