Core B will have three specific aims.
Specific Aim 1 is to harvest, characterize and propagate human lung microvascular endothelial cells from subjects undergoing lobectomy or pneumonectomy at MCG Health Inc.;provide said cells to investigators in all four projects for permeability, analytical and other molecular studies.
Specific Aim 2 is to create and supply to all four projects mice with ALI induced by intra-tracheal instillation of gram positive or gram negative bacterial products.
Specific Aim 3 is to perform in vivo lung capillary permeability (Evan's Blue Dye Method) measurements and lung function (airway mechanics) measurements for studies designed by each of the four projects.

Public Health Relevance

The goal of this Core is to help the four studies develop new approaches to the management of acute lung injury and acute respiratory distress syndrome, conditions that carry a high degree of mortality. It will provide the tools needed to investigate means of restoring the known dysfunction and increased permeability of lung capillary vessels.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL101902-02
Application #
8376424
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2012-07-01
Project End
2016-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
2
Fiscal Year
2012
Total Cost
$390,000
Indirect Cost
$130,000
Name
Georgia Regents University
Department
Type
DUNS #
966668691
City
Augusta
State
GA
Country
United States
Zip Code
30912
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