Core B will have three specific aims.
Specific Aim 1 is to harvest, characterize and propagate human lung microvascular endothelial cells from subjects undergoing lobectomy or pneumonectomy at MCG Health Inc.;provide said cells to investigators in all four projects for permeability, analytical and other molecular studies.
Specific Aim 2 is to create and supply to all four projects mice with ALI induced by intra-tracheal instillation of gram positive or gram negative bacterial products.
Specific Aim 3 is to perform in vivo lung capillary permeability (Evan's Blue Dye Method) measurements and lung function (airway mechanics) measurements for studies designed by each of the four projects.
The goal of this Core is to help the four studies develop new approaches to the management of acute lung injury and acute respiratory distress syndrome, conditions that carry a high degree of mortality. It will provide the tools needed to investigate means of restoring the known dysfunction and increased permeability of lung capillary vessels.
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|Aggarwal, Saurabh; Gross, Christine M; Rafikov, Ruslan et al. (2014) Nitration of tyrosine 247 inhibits protein kinase G-1? activity by attenuating cyclic guanosine monophosphate binding. J Biol Chem 289:7948-61|
|Gonzales, Joyce N; Gorshkov, Boris; Varn, Matthew N et al. (2014) Protective effect of adenosine receptors against lipopolysaccharide-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol 306:L497-507|
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|Gross, Christine M; Aggarwal, Saurabh; Kumar, Sanjiv et al. (2014) Sox18 preserves the pulmonary endothelial barrier under conditions of increased shear stress. J Cell Physiol 229:1802-16|
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|Czikora, István; Alli, Abdel; Bao, Hui-Fang et al. (2014) A novel tumor necrosis factor-mediated mechanism of direct epithelial sodium channel activation. Am J Respir Crit Care Med 190:522-32|
|Chen, Feng; Kumar, Sanjiv; Yu, Yanfang et al. (2014) PKC-dependent phosphorylation of eNOS at T495 regulates eNOS coupling and endothelial barrier function in response to G+ -toxins. PLoS One 9:e99823|
|Gonzales, Joyce N; Kim, Kyung-mi; Zemskova, Marina A et al. (2014) Low anticoagulant heparin blocks thrombin-induced endothelial permeability in a PAR-dependent manner. Vascul Pharmacol 62:63-71|
|Rafikov, Ruslan; Dimitropoulou, Christiana; Aggarwal, Saurabh et al. (2014) Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA. J Biol Chem 289:4710-22|
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