Instmctions): (Core B, Lauer) The Shared Resource Core is one of the three cores required by the Cleveland Clinic Program of Excellence in Glycoscience (CC-PEG). The primary objectives of this Core are: 1) to accelerate the pace of the individual research projects by providing them with the glycoscience skills and facilities necessary for glycosaminoglycan analysis, and 2) to provide post-doctoral fellows supported by this program with hands-on instruction in glycosaminoglycan analytical techniques as part of a close partnership with the Glycoscience Skills Development Core. The Shared Resource Core will provide services and instruction in a variety of analytical techniques, involving glycosaminoglycans: (a) extraction and purification, (b) mass, size and sulfation profiling, (c) binding partners and modifications, (d) immunohistochemistry and gene expression, and (e) smooth muscle/endothellal cell culture. These services and instructions will insure timeliness, efficiency and uniformity for all the projects in achieving their aims that require extensive analyses of glycosaminoglycans/proteoglycans, thereby facilitating meeting the overall goals of the CC-PEG to determine the roles of hyaluronan matrices in vascular pathologies.

Public Health Relevance

The Shared Resource Core will contribute to public health by providing the Individual research projects with the specialized analytical tools required for glycosaminoglycan/proteoglycan analyses. These analyses will involve clinical samples from patients with vascular disease involving diabetes, pulmonary hypertension and inflammatory bowel disease.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL107147-04
Application #
8669092
Study Section
Special Emphasis Panel (ZHL1-CSR-H)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
4
Fiscal Year
2014
Total Cost
$256,496
Indirect Cost
$88,264
Name
Cleveland Clinic Lerner
Department
Type
DUNS #
135781701
City
Cleveland
State
OH
Country
United States
Zip Code
44195
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