The Mouse Management Component supports 2 full-time animal technician position equivalents (in practice, approximately one half-time position for each project). These individuals are fully trained and engaged in the maintenance of gene-targeted and transgenic mouse models, as proposed in the individual projects. This component has the necessary equipment and space in a pathogen-free mouse-specific vivarium, as detailed below. The actual production of new mouse models will be done through the currently established UCSD Transgenic Mouse and Embryonic Stem Cell Gene Targeting Core (for details, please see the web site http://cancer.ucsd.edu/Research/Shared/tgm/default2.asp). This state-of-the-art facility has an excellent track record in the production of genetically altered mice. Thus, rather than doing gene targeting ourselves, it makes sense for the PEG to pay this established UCSD Core a fee-for-service for the generation of mice. While the total costs are about the same, we do avoid duplicative functions at the institution. Our past experience with this approach has been good. We will of course also take advantage of the ongoing targeting projects at the Knockout Mouse Project (KOMP) (www.komp.org/) Based on our experience to date in similar projects, we also feel it is best to keep the daily management and breeding of mice, the animal technicians involved, the costs of mouse cage space, and the use of approaches such as Speed Congenics as Core functions?rather than allocating resources to each project for these activities. This approach also reduces waste and allows the maximum flexibility in accommodating the needs of all the projects, which will vary from year to year While this makes for a rather large budget for an """"""""Administrative Core,"""""""" most of the budget is actually for mouse-related costs relevant to all projects. Almost all of the specific aims of all of the projects and the functions of the other Cores require the use of mice, which will be managed by Core C. Likewise, the administrative component is important for all Projects and Cores. Thus. Core C will effectively support all of the other Projects and Cores.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL107150-02
Application #
8375514
Study Section
Special Emphasis Panel (ZHL1-CSR-H)
Project Start
Project End
Budget Start
2012-06-01
Budget End
2013-05-31
Support Year
2
Fiscal Year
2012
Total Cost
$636,770
Indirect Cost
$225,840
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Gordts, Philip L S M; Nock, Ryan; Son, Ni-Huiping et al. (2016) ApoC-III inhibits clearance of triglyceride-rich lipoproteins through LDL family receptors. J Clin Invest 126:2855-66
Varki, Ajit (2016) Biological Roles of Glycans. Glycobiology :
Yamaguchi, Masaya; Hirose, Yujiro; Nakata, Masanobu et al. (2016) Evolutionary inactivation of a sialidase in group B Streptococcus. Sci Rep 6:28852
Secundino, Ismael; Lizcano, Anel; Roupé, K Markus et al. (2016) Host and pathogen hyaluronan signal through human siglec-9 to suppress neutrophil activation. J Mol Med (Berl) 94:219-33
Thacker, Bryan E; Seamen, Emylie; Lawrence, Roger et al. (2016) Expanding the 3-O-Sulfate Proteome--Enhanced Binding of Neuropilin-1 to 3-O-Sulfated Heparan Sulfate Modulates Its Activity. ACS Chem Biol 11:971-80
Zhang, Ling-Juan; Sen, George L; Ward, Nicole L et al. (2016) Antimicrobial Peptide LL37 and MAVS Signaling Drive Interferon-β Production by Epidermal Keratinocytes during Skin Injury. Immunity 45:119-30
Schwarz, Flavio; Springer, Stevan A; Altheide, Tasha K et al. (2016) Human-specific derived alleles of CD33 and other genes protect against postreproductive cognitive decline. Proc Natl Acad Sci U S A 113:74-9
Sato, Emi; Muto, Jun; Zhang, Ling-Juan et al. (2016) The Parathyroid Hormone Second Receptor PTH2R and its Ligand Tuberoinfundibular Peptide of 39 Residues TIP39 Regulate Intracellular Calcium and Influence Keratinocyte Differentiation. J Invest Dermatol 136:1449-59
Miles, L A; Baik, N; Lighvani, S et al. (2016) Deficiency of Plasminogen Receptor, Plg-RKT, Causes Defects in Plasminogen Binding and Inflammatory Macrophage Recruitment in vivo. J Thromb Haemost :
Zaiss, Anne K; Foley, Erin M; Lawrence, Roger et al. (2016) Hepatocyte Heparan Sulfate Is Required for Adeno-Associated Virus 2 but Dispensable for Adenovirus 5 Liver Transduction In Vivo. J Virol 90:412-20

Showing the most recent 10 out of 59 publications