OVERVIEW OF GLYCAN ANALYSIS COMPONENT: Glycans play key roles in biological processes relevant to inflammation, such as cell-cell recognition, cell migration, and signal transduction. In order to understand glycan function in inflammation, it is essential to have an in-depth knowledge of glycan structural diversity in various cell types. Because enormous structural diversity exists in naturally occurring glycans, it has proven difficult to devise a universal protocol for glycan analysis. Moreover, sample complexity makes the isolation and purification of cell surface glycans a major methodological challenge. The analytical component of Core B will work within the framework of the Glycotechnology Core Resource at UCSD, a well-known center for providing specialized glycan analysis (glycotech.ucsd.edu). The Glycotechnology Core Resource was established in 1993 and since then has provided analytical support to a large number of investigators. Over the years, the Resource has developed skills to isolate, purify and structurally characterize polysaccharides or oligosaccharides from samples with limited amounts of analyte in the presence of a large background of complex biological contaminants. The state-of-the-art instrumentation coupled with many years of analytical experience will ensure that the analytic needs of the proposed Projects will be met. Besides carrying out custom analyses, the Resource has also trained graduate students, postdoctoral fellows, and principal investigators in glycan analytical techniques. Thus, the analytical group of Core B will also participate In the training program described in Core A, as well as perform glycan analyses for the individual Projects. Table 1 describes analytical techniques that will be used by the Projects.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL107150-03
Application #
8477266
Study Section
Special Emphasis Panel (ZHL1-CSR-H)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
3
Fiscal Year
2013
Total Cost
$216,587
Indirect Cost
$43,093
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Patras, Kathryn A; Nizet, Victor (2018) Group B Streptococcal Maternal Colonization and Neonatal Disease: Molecular Mechanisms and Preventative Approaches. Front Pediatr 6:27
Raitman, Irene; Huang, Mia L; Williams, Selwyn A et al. (2018) Heparin-fibronectin interactions in the development of extracellular matrix insolubility. Matrix Biol 67:107-122
Dokoshi, Tatsuya; Zhang, Ling-Juan; Nakatsuji, Teruaki et al. (2018) Hyaluronidase inhibits reactive adipogenesis and inflammation of colon and skin. JCI Insight 3:
Miles, L A; Baik, N; Bai, H et al. (2018) The plasminogen receptor, Plg-RKT, is essential for mammary lobuloalveolar development and lactation. J Thromb Haemost 16:919-932
Qiu, Hong; Shi, Songshan; Yue, Jingwen et al. (2018) A mutant-cell library for systematic analysis of heparan sulfate structure-function relationships. Nat Methods 15:889-899
Godula, Kamil (2018) Following sugar patterns in search of galectin function. Proc Natl Acad Sci U S A 115:2548-2550
Huang, Mia L; Michalak, Austen L; Fisher, Christopher J et al. (2018) Small Molecule Antagonist of Cell Surface Glycosaminoglycans Restricts Mouse Embryonic Stem Cells in a Pluripotent State. Stem Cells 36:45-54
Huang, Mia L; Tota, Ember M; Verespy 3rd, Stephen et al. (2018) Glycocalyx Scaffolding to Control Cell Surface Glycan Displays. Curr Protoc Chem Biol 10:e40
Ge, Xiao Na; Bastan, Idil; Ha, Sung Gil et al. (2018) Regulation of eosinophil recruitment and allergic airway inflammation by heparan sulfate proteoglycan (HSPG) modifying enzymes. Exp Lung Res 44:98-112
Gordts, Philip L S M; Esko, Jeffrey D (2018) The heparan sulfate proteoglycan grip on hyperlipidemia and atherosclerosis. Matrix Biol 71-72:262-282

Showing the most recent 10 out of 110 publications