; The surgical/imaging cores (Core B) will provide the expertise and quality control for surgical models in evaluation of the signaling pathways associated with arteriogenesis, preparation of animals for imaging,performing multi-modality imaging, and coordinating image analyses for all projects. Recently renovated Yale facilities are uniquely capable of conducting advanced imaging studies on acute and chronically instrumented animals. The small animal surgical/imaging core includes capacity for anesthesia, hemodynamic monitoring, microsurgery, dedicated animal housing, facilities for preparation of ex vivo specimen and contrast, and an imaging suite that includes high-resolution (25-100 um) in vivo microCT scanners, and ultra-high resolution (8 um) specimen microCT, deep muscle laser Doppler imaging system, and scanning laser Doppler imager. The surgical core will provide two well established murine models of hindlimb ischemia, and a vasoreactivity model. Previous studies have suggested the importance of standardized surgical interventions with minimal trauma to the animals. To assure uniformity of the experimental models between projects an experienced surgical team will perform all of the surgical interventions and peri-operative care. The imaging core will serve as a central resource for;preparation of specimens and contrast agents, animal preparation and monitoring for in vivo imaging, image acquisition and reconstruction, image archive, and will assist with the supervision of registration of the image data derived from multiple imaging systems and image quantification. The imaging core will include individuals with unique technical qualifications and sufficient dedicated time to assure consistent high quality images. Registration and analysis of images from multiple imaging systems will be provided by individuals with computer and bioengineering expertise supervised by faculty from Departments of Medicine, Diagnostic Radiology, and Biomedical Engineering with recognized expertise in the imaging sciences. This surgical/imaging team will be supervised by Dr. Sinusas, who will serve as the director of Core B. This team will advise participating investigators regarding the appropriate application of animal models and imaging.
The discovery of a signaling cascade controlling growth of new arteries will provide new insights and tools into developing new therapeutic strategies for treatment of atherosclerotic cardiovascular diseases including coronary, cerebral and peripheral vascular diseases.
|Kofler, Natalie; Simons, Michael (2016) The expanding role of neuropilin: regulation of transforming growth factor-Î² and platelet-derived growth factor signaling in the vasculature. Curr Opin Hematol 23:260-7|
|Sawyer, Andrew J; Kyriakides, Themis R (2016) Matricellular proteins in drug delivery: Therapeutic targets, active agents, and therapeutic localization. Adv Drug Deliv Rev 97:56-68|
|Baeyens, Nicolas; Bandyopadhyay, Chirosree; Coon, Brian G et al. (2016) Endothelial fluid shear stress sensing in vascular health and disease. J Clin Invest 126:821-8|
|Chen, Pei-Yu; Simons, Michael (2016) When endothelial cells go rogue. EMBO Mol Med 8:1-2|
|Kristofik, Nina; Calabro, Nicole E; Tian, Weiming et al. (2016) Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice. Blood 128:1642-50|
|Baeyens, Nicolas; LarrivÃ©e, Bruno; Ola, Roxana et al. (2016) Defective fluid shear stress mechanotransduction mediates hereditary hemorrhagic telangiectasia. J Cell Biol 214:807-16|
|Siragusa, Mauro; FrÃ¶hlich, Florian; Park, Eon Joo et al. (2015) Stromal cell-derived factor 2 is critical for Hsp90-dependent eNOS activation. Sci Signal 8:ra81|
|Bancroft, Tara; Bouaouina, Mohamed; Roberts, Sophia et al. (2015) Up-regulation of thrombospondin-2 in Akt1-null mice contributes to compromised tissue repair due to abnormalities in fibroblast function. J Biol Chem 290:409-22|
|Eelen, Guy; de Zeeuw, Pauline; Simons, Michael et al. (2015) Endothelial cell metabolism in normal and diseased vasculature. Circ Res 116:1231-44|
|Simons, Michael; Eichmann, Anne (2015) Molecular controls of arterial morphogenesis. Circ Res 116:1712-24|
Showing the most recent 10 out of 44 publications