Project II is designed to discover the molecular basis of CFTR-mediated restraint of ENaC activity in human airway epithelia. CFTR-mediated chloride secretion and ENaC-mediated sodium absorption have been considered central to control of airway surface liquid hydration, but the concept of reciprocal control of these opposing ion transport pathways is controversial. In some tissues where CFTR and ENaC are each expressed, their stimulation is coordinated to achieve Na and Cl absorption. Indeed, that is the case in alveolar type 2 cells (AT2C), which are to be characterized in Project III of this PPG. However, in airway epithelia, abundant evidence suggests that CFTR restrains ENaC function. We recently reported that CFTR and ENaC coimmunoprecipitate in normal airway epithelia, and ENaC in CF bronchial epithelium undergoes more extensive cleavage than ENaC in normal. Because ENaC mediated Na+ absorption is activated by cleavage of ENaC extracellular domains, this finding is congruent with the notion that CFTR restrains ENaC cleavage in normal bronchial epithelia. Project II proposes to identify the molecular basis of CFTR-ENaC associations in airway epithelia, which will be accomplished by biochemical assays, comparisons of CFTR and ENaC associated proteins in bronchial airway cells and AT2C, and molecular modeling of detected interactions (Aim 1). With this information. Project II will test two mechanistic hypotheses that describe inhibition of ENaC cleavage.
In Aim 2, we hypothesize that physical interaction of the R-domain of CFTR with the cytosolic N-termini of ENaC inhibits ENaC proteolysis and activation. This hypothesis is based on the novel observation that ENaC proteolysis is stimulated by phosphoinositde-binding of its cytosolic N terminal tails.
In Aim 3, Project II will test the requirement for CFTR function in the regulation of ENaC cleavage, and will further test specifically how CFTR's control of ASL pH mediates CFTR-specific effects on ENaC proteolysis. Information from these aims will further our understanding of CFTR regulation of ENaC function, specifically addressing the questions of why this regulation is cell specific, and yet when present, how it may be mediated by multiple mechanisms.
Airway surfaces are protected by a hydrated layer of salt, water and mucins. Project II addresses control of surface liquid hydration by the ion channels CFTR and ENaC. In cytic fibrosis airway disease, CFTR is absent, ENaC is unregulated and airway surface hydration is lost. Airway obstruction follows, and this process may occur in other respiratory diseases, such as smoking induced COPD. Our results will expand understanding of and potential treatments for human lung disease.
|Abdullah, Lubna H; Coakley, Raymond; Webster, Megan J et al. (2018) Mucin Production and Hydration Responses to Mucopurulent Materials in Normal versus Cystic Fibrosis Airway Epithelia. Am J Respir Crit Care Med 197:481-491|
|Yu, Dongfang; Saini, Yogesh; Chen, Gang et al. (2018) Loss of ? Epithelial Sodium Channel Function in Meibomian Glands Produces Pseudohypoaldosteronism 1-Like Ocular Disease in Mice. Am J Pathol 188:95-110|
|Rowson-Hodel, A R; Wald, J H; Hatakeyama, J et al. (2018) Membrane Mucin Muc4 promotes blood cell association with tumor cells and mediates efficient metastasis in a mouse model of breast cancer. Oncogene 37:197-207|
|Terryah, Shawn T; Fellner, Robert C; Ahmad, Saira et al. (2018) Evaluation of a SPLUNC1-derived peptide for the treatment of cystic fibrosis lung disease. Am J Physiol Lung Cell Mol Physiol 314:L192-L205|
|Muhlebach, Marianne S; Zorn, Bryan T; Esther, Charles R et al. (2018) Initial acquisition and succession of the cystic fibrosis lung microbiome is associated with disease progression in infants and preschool children. PLoS Pathog 14:e1006798|
|Shobair, Mahmoud; Popov, Konstantin I; Dang, Yan L et al. (2018) Mapping allosteric linkage to channel gating by extracellular domains in the human epithelial sodium channel. J Biol Chem 293:3675-3684|
|Kota, Pradeep; Gentzsch, Martina; Dang, Yan L et al. (2018) The N terminus of ?-ENaC mediates ENaC cleavage and activation by furin. J Gen Physiol 150:1179-1187|
|Livraghi-Butrico, Alessandra; Wilkinson, Kristen J; Volmer, Allison S et al. (2018) Lung disease phenotypes caused by overexpression of combinations of ?-, ?-, and ?-subunits of the epithelial sodium channel in mouse airways. Am J Physiol Lung Cell Mol Physiol 314:L318-L331|
|Chen, Gang; Volmer, Allison S; Wilkinson, Kristen J et al. (2018) Role of Spdef in the Regulation of Muc5b Expression in the Airways of Naive and Mucoobstructed Mice. Am J Respir Cell Mol Biol 59:383-396|
|Livraghi-Butrico, A; Grubb, B R; Wilkinson, K J et al. (2017) Contribution of mucus concentration and secreted mucins Muc5ac and Muc5b to the pathogenesis of muco-obstructive lung disease. Mucosal Immunol 10:829|
Showing the most recent 10 out of 56 publications