Nucleotides and nucleosides in the ainway surface liquid (ASL) activate cell surface purinergic receptors that critically regulate ainway epithelial fluid secretion and mucociliary clearance (l /!CC) functions. Nucleotide release rates and nucleotide/nucleoside levels in ASL are finely tuned to sustain MCC activities without promoting excessive fluid secretion or inflammation. However, the mechanisms of ainway epithelial nucleotide release are incompletely understood. Studies in our lab have revealed that goblet and ciliated cells release nucleotides via exocytotic and conductive pathways, but there are important gaps in identifying/characterizing key components of these pathways. Our recent results provide insights into ATP uptake, storage, and release from goblet cell mucin granules. Our data suggest that the vesicular nucleotide transporter (VNUT) SLC17A9 regulates the uptake of ATP into mucin granules, and therefore contributes to mucin granule secretion-associated ATP release. Our results also suggest that the plasma membrane channel pannexin 1 mediates ATP release from ciliated cells, and that pannexin 1 activity is controlled by TRPV4 and Rho/ROCK activation. A plan to define the contribution of VNUT and pannexin 1 to nucleotide release from ainway epithelia is outlined in this project by pursuing the following Specific Aims: (1) to test the hypothesis that VNUT contributes to mucin granule secretion-associated nucleotide release, and (2) to test the hypothesis that pannexin 1 channels mediate ATP release from ciliated cell-rich ainway epithelia. We will utilize RNA interference techniques and gene targeting approaches in in vitro and in vivo models of airway epithelia, respectively, to assess (a) the extent to which VNUT contributes to nucleotide transport into mucin granules and to nucleotide release from mucin secreting epithelia, and (b) to define the role of pannexin 1 and upstream effectors in nucleotide release in the ASL. We will further explore the effect of VNUT and pannexin 1 gene deletions in the mucociliary and alveolar liquid clearance defects that have been attributed to unbalanced nucleotide release in asthma and viral infected lungs. Completion of these studies will establish molecular mechanism(s) for the physiologically important process of nucleotide release, and may delineate novel pharmacological strategies aimed to treat lung diseases associated with abnormal mucus clearance and inflammatory lung diseases.

Public Health Relevance

This project proposes to study two novel regulatory pathways of nucleotide release and their involvement in lung diseases and identify new therapeutic approaches to treat abnormal liquid clearance and inflammatory lung diseases. Nucleotides and adenosine in the ASL are critical regulators of ainway mucociliary and liquid clearance. Imbalance of these activities is at the pathogenesis core of obstructive and inflammatory lung diseases, which are responsible for high morbidity and lethality in the United States.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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University of North Carolina Chapel Hill
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Donoghue, Lauren J; Livraghi-Butrico, Alessandra; McFadden, Kathryn M et al. (2017) Identification of trans Protein QTL for Secreted Airway Mucins in Mice and a Causal Role for Bpifb1. Genetics 207:801-812
Sandefur, Conner I; Boucher, Richard C; Elston, Timothy C (2017) Mathematical model reveals role of nucleotide signaling in airway surface liquid homeostasis and its dysregulation in cystic fibrosis. Proc Natl Acad Sci U S A 114:E7272-E7281
Wang, Ling; Ariyarathna, Yamuna; Ming, Xin et al. (2017) A Novel Family of Small Molecules that Enhance the Intracellular Delivery and Pharmacological Effectiveness of Antisense and Splice Switching Oligonucleotides. ACS Chem Biol 12:1999-2007
Livraghi-Butrico, Alessandra; Grubb, Barbara R; Wilkinson, Kristen J et al. (2017) Contribution of mucus concentration and secreted mucins Muc5ac and Muc5b to the pathogenesis of muco-obstructive lung disease. Mucosal Immunol 10:395-407
Sesma, Juliana I; Weitzer, Clarissa D; Livraghi-Butrico, Alessandra et al. (2016) UDP-glucose promotes neutrophil recruitment in the lung. Purinergic Signal 12:627-635
Esther Jr, Charles R; Turkovic, Lidija; Rosenow, Tim et al. (2016) Metabolomic biomarkers predictive of early structural lung disease in cystic fibrosis. Eur Respir J 48:1612-1621
Dickey, Audrey S; Pineda, Victor V; Tsunemi, Taiji et al. (2016) PPAR-? is repressed in Huntington's disease, is required for normal neuronal function and can be targeted therapeutically. Nat Med 22:37-45
Yu, Dongfang; Davis, Richard M; Aita, Megumi et al. (2016) Characterization of Rat Meibomian Gland Ion and Fluid Transport. Invest Ophthalmol Vis Sci 57:2328-43
Shobair, Mahmoud; Dagliyan, Onur; Kota, Pradeep et al. (2016) Gain-of-Function Mutation W493R in the Epithelial Sodium Channel Allosterically Reconfigures Intersubunit Coupling. J Biol Chem 291:3682-92
Kirby, Brett S; Schwarzbaum, Pablo J; Lazarowski, Eduardo R et al. (2015) Liberation of ATP secondary to hemolysis is not mutually exclusive of regulated export. Blood 125:1844-5

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