The proteomic core will provide cutting edge discovery protein biochemistry and proteomics tools to address key mechanistic questions, in support ofthe underlying concept that disease-induced changes in autophagy lead to alterations in protein quantity, abundance of specific isoforms and post-translational modifications, which drive cellular phenotype. The core will provide discovery based approaches for protein quantification and the identification of post-translational modifications including phosphorylation, 0-GlcNAcylation and cysteine oxidative modifications for the detailed analysis of a number of subproteomes and protein complexes. The core will employ its recently modified method that allows the site specific quantification and comparison of S-nitrosation, S-glutathionylation and sulfonic acid, the three reversible cysteine modifications. As well, the core will provide multiplex assays for the absolute quantification of key proteins involved in autophagy, lysosomal function, mitochondrial biogenesis and nutrient sensing using multiple reaction monitoring, a targeted mass spectrometry based method. The panels developed will quantify the total protein concentration as well as differentiate between the various protein isoforms which arise from different genes or splice variants of a single gene and for a subset, specific regulatory posttranslational modification.

Public Health Relevance

The purpose of the Protein Chemistry and Proteomic Core (Core B) is to apply cutting edge discovery protein biochemistry and proteomics tools to address key mechanistic questions from each PPG project. Core B will provide crucial experimental services in support of the underlying concept that disease-induced changes in protein quantify and PTMs dictate the underlying autophagy status, its regulation by different signaling events and ultimately myocyte function. Being able to therapeutically manipulate autophagy will allow us to address protection and heart failure.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
7P01HL112730-02
Application #
8683230
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2014-07-21
Budget End
2015-06-30
Support Year
2
Fiscal Year
2014
Total Cost
$262,313
Indirect Cost
$108,011
Name
Cedars-Sinai Medical Center
Department
Type
DUNS #
075307785
City
Los Angeles
State
CA
Country
United States
Zip Code
90048
Pepe, Salvatore; Mentzer Jr, Robert M; Gottlieb, Roberta A (2014) Cell-permeable protein therapy for complex I dysfunction. J Bioenerg Biomembr 46:337-45
Andres, Allen M; Hernandez, Genaro; Lee, Pamela et al. (2014) Mitophagy is required for acute cardioprotection by simvastatin. Antioxid Redox Signal 21:1960-73
Linton, Phyllis-Jean; Thoman, Marilyn L (2014) Immunosenescence in monocytes, macrophages, and dendritic cells: lessons learned from the lung and heart. Immunol Lett 162:290-7
Hernandez, Genaro; Thornton, Christine; Stotland, Aleksandr et al. (2013) MitoTimer: a novel tool for monitoring mitochondrial turnover. Autophagy 9:1852-61
Ferree, Andrew W; Trudeau, Kyle; Zik, Eden et al. (2013) MitoTimer probe reveals the impact of autophagy, fusion, and motility on subcellular distribution of young and old mitochondrial protein and on relative mitochondrial protein age. Autophagy 9:1887-96
Riehle, Christian; Wende, Adam R; Sena, Sandra et al. (2013) Insulin receptor substrate signaling suppresses neonatal autophagy in the heart. J Clin Invest 123:5319-33