Core B is the ROS Analytical Core. This is a new core that became necessary because all the projects require quantitative measurement of reactive oxygen species (ROS). Measuring ROS is challenging, particularly when done in vivo, because of multiple intracellular sources of ROS in cells and the known propensity of one form of ROS to readily transform into another. One overriding facet of oxidative stress, however, is peroxidation of unsaturated fatty acid moieties of neutral lipids and phospholipids in cell membranes and oxidation of cellular proteins. Moreover, measuring specific ROS may not explain the functional outcomes in pathological pain and related pathogenesis. An assessment of ROS-induced lipid peroxidation and oxidation of proteins appears to be a logical choice for understanding the underlying mechanism in our pain model. The findings of lipid peroxidation and oxidation proteins will be supported by the real-time live cell and histochemical fixed cell imaging to be conducted in collaboration with Core C. Therefore, ROS Analytical Core will measure lipid peroxidation products and oxidized proteins, and ROS generation in live or fixed cells. The ROS Analytical Core is composed of three branches with an assigned Co-Director for each: 1) Lipid Peroxidation Analysis, 2) Protein Oxidation Analysis, and 3) Histopathology branches. While branches 1) and 2) are for the chemical analysis, branch 3) is to measure intracellular ROS levels using both real-time live-cell imaging and histochemical imaging from fixed cells. For the use of the imaging facility, this core works very closely with Core C (Imaging Core) and the Co-Director of Histopathology of this core is also a Co-Director of the Imaging Core. Therefore, the ROS Analytical Core becomes an integral part of the PPG in delineating the role of ROS in central and peripheral sensitization in pain.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS011255-36
Application #
8375575
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
2014-03-31
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
36
Fiscal Year
2012
Total Cost
$129,662
Indirect Cost
$26,822
Name
University of Texas Medical Br Galveston
Department
Type
DUNS #
800771149
City
Galveston
State
TX
Country
United States
Zip Code
77555
Carter, Michael W; Johnson, Kathia M; Lee, Jun Yeon et al. (2016) Comparison of Mechanical Allodynia and Recovery of Locomotion and Bladder Function by Different Parameters of Low Thoracic Spinal Contusion Injury in Rats. Korean J Pain 29:86-95
Young, E E; Bryant, C D; Lee, S E et al. (2016) Systems genetic and pharmacological analysis identifies candidate genes underlying mechanosensation in the von Frey test. Genes Brain Behav 15:604-15
Hammell, D C; Zhang, L P; Ma, F et al. (2016) Transdermal cannabidiol reduces inflammation and pain-related behaviours in a rat model of arthritis. Eur J Pain 20:936-48
Yuan, Su-Bo; Ji, Guangchen; Li, Bei et al. (2015) A Wnt5a signaling pathway in the pathogenesis of HIV-1 gp120-induced pain. Pain 156:1311-9
Ji, Guangchen; Li, Zhen; Neugebauer, Volker (2015) Reactive oxygen species mediate visceral pain-related amygdala plasticity and behaviors. Pain 156:825-36
Neugebauer, Volker (2015) Amygdala pain mechanisms. Handb Exp Pharmacol 227:261-84
Ji, Guangchen; Neugebauer, Volker (2014) CB1 augments mGluR5 function in medial prefrontal cortical neurons to inhibit amygdala hyperactivity in an arthritis pain model. Eur J Neurosci 39:455-66
Hassler, Shayne N; Johnson, Kathia M; Hulsebosch, Claire E (2014) Reactive oxygen species and lipid peroxidation inhibitors reduce mechanical sensitivity in a chronic neuropathic pain model of spinal cord injury in rats. J Neurochem 131:413-7
Medina, Georgina; Ji, Guangchen; Gr├ęgoire, St├ęphanie et al. (2014) Nasal application of neuropeptide S inhibits arthritis pain-related behaviors through an action in the amygdala. Mol Pain 10:32
Kiritoshi, Takaki; Sun, Hao; Ren, Wenjie et al. (2013) Modulation of pyramidal cell output in the medial prefrontal cortex by mGluR5 interacting with CB1. Neuropharmacology 66:170-8

Showing the most recent 10 out of 585 publications