The machine shop in the Department of Neurobiology currently consists of two lathes, a milling machine, a bandsaw and two drill presses. It is managed by Mike Lafratta. This core will bring together Mike's forty years of experience he has in building and designing neurobiological equipment, offering more specified techniques that could ever be supplied by a machine shop serving the school's departments. His experience is not limited to building metallic or plastic machinery, for he has taken advantage of years of development of equipment by a large number of exceptional scientists. Many of the devices he makes have gone through many model adjustments over the years to suit the department's needs. It is rare when he requires anything but a rough sketch to design what is needed for a lab. Due to his expertise, he is able to make useful suggestions as to improvements of a particular design. Additionally, he offers knowledge of solving and fixing and mechanical problem encountered in the labs. During the past years, he has provided invaluable service to the numbers of the program project grant by building and adapting countless custom optical components, microscopes for use with brain slices, adaptors for manipulators, perfusion setups, temperature control apparatus, recording chambers and countless other contributions to the research programs of Drs. Bean Clapham, Fishbach and Regehr. The machine shop will be one of the most extensively used by the Core members as they begin new projects related to this program project grant.

Project Start
1999-12-01
Project End
2000-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
2
Fiscal Year
2000
Total Cost
$395,552
Indirect Cost
Name
Harvard University
Department
Type
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115
Richards, Kathryn S; Swensen, Andrew M; Lipscombe, Diane et al. (2007) Novel CaV2.1 clone replicates many properties of Purkinje cell CaV2.1 current. Eur J Neurosci 26:2950-61
McDonough, Stefan I; Mori, Yasuo; Bean, Bruce P (2005) FPL 64176 modification of Ca(V)1.2 L-type calcium channels: dissociation of effects on ionic current and gating current. Biophys J 88:211-23
Blitz, Dawn M; Regehr, Wade G (2003) Retinogeniculate synaptic properties controlling spike number and timing in relay neurons. J Neurophysiol 90:2438-50
Blair, Nathaniel T; Bean, Bruce P (2003) Role of tetrodotoxin-resistant Na+ current slow inactivation in adaptation of action potential firing in small-diameter dorsal root ganglion neurons. J Neurosci 23:10338-50
Do, Michael Tri H; Bean, Bruce P (2003) Subthreshold sodium currents and pacemaking of subthalamic neurons: modulation by slow inactivation. Neuron 39:109-20
Martina, Marco; Yao, Gui Lan; Bean, Bruce P (2003) Properties and functional role of voltage-dependent potassium channels in dendrites of rat cerebellar Purkinje neurons. J Neurosci 23:5698-707
Swensen, Andrew M; Bean, Bruce P (2003) Ionic mechanisms of burst firing in dissociated Purkinje neurons. J Neurosci 23:9650-63
Mitterdorfer, Jorg; Bean, Bruce P (2002) Potassium currents during the action potential of hippocampal CA3 neurons. J Neurosci 22:10106-15
Blair, Nathaniel T; Bean, Bruce P (2002) Roles of tetrodotoxin (TTX)-sensitive Na+ current, TTX-resistant Na+ current, and Ca2+ current in the action potentials of nociceptive sensory neurons. J Neurosci 22:10277-90
Chen, Chinfei; Blitz, Dawn M; Regehr, Wade G (2002) Contributions of receptor desensitization and saturation to plasticity at the retinogeniculate synapse. Neuron 33:779-88

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