An advantage of conducting research within a coordinated Program Project is the ability to create core facilities to (i) efficiently and economically share resources and administration support, (ii) provide infrastructure for the scientific objectives of the program, (iii) provide mechanisms for disseminating results and facilitating discussions within the group, and (iv) coordinate interactions with outside investigators to obtain critical evaluation, technical advice and intellectual input to keep the work on track and at the cutting edge of technologies in the field. To this end an Analytical, Administrative and Animal/Reagent Core (Core A) will be created and directed by Dr. Christine Gall. Core A will address 4 specific aims.
Aim 1 will be to maintain an Analytical Core for Microscopy, Electrophysiology, Behavior and Assay functions. Studies within Projects 1-4 entail analyses of long term potentiation (LTP) in hippocampal slices, localized signaling to actin, modulating endogenous BDNF protein content, and evaluation of treatment effects on unsupervised learning. This will be accomplished using Analytical Core facilities and personnel for microscopy-image analysis, electrophysiology, behavioral analysis (unsupervised learning) and protein assays.
Aim 2 will be to support Animal and Reagent functions. Core personnel will coordinate purchases of reagents and ampakines, support mouse colonies employed for UCl Projects, and perform genotyping.
Aim 3 will be to manage collaborations and integration of research among Project laboratories, and provide input from internal and external advisory boards (Administration). This includes coordinating both research activities among the projects for access to key analytical facilities (e.g., microscopic, electrophysiological and behavioral facilities and staff) and Program Project collaborative meetings. The Core will also convene meetings with Internal and External Advisory Boards, and seminar speakers.
Aim 4 is to provide general administrative support and computer assistance for all program investigators (Administration). This includes general administrative support for grants management, coordinating seminars, oversight of animal use in Core A, supervision of Core personnel, and maintenance of computer servers for Program activities. Overall the Core facilities and functions will provide critical integration of research within the Projects and will support technical platforms that are critical for reaching project goals.

Public Health Relevance

The program project will test if there are common neurobiological processes underlying cognitive impairments in different animal models and if up-regulating BDNF is a broadly effective therapeutic strategy. To meet these program goals is critical that the different subprojects use the same treatments and measures. The Core will assure this uniformity and will provide mechanisms for sharing critical analytical facilities, technical expertise, and research advances throughout the program.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS045260-11
Application #
8723901
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
11
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of California Irvine
Department
Type
DUNS #
City
Irvine
State
CA
Country
United States
Zip Code
92697
Bi, Xiaoning; Sun, Jiandong; Ji, Angela X et al. (2016) Potential therapeutic approaches for Angelman syndrome. Expert Opin Ther Targets 20:601-13
Baudry, Michel; Bi, Xiaoning (2016) Calpain-1 and Calpain-2: The Yin and Yang of Synaptic Plasticity and Neurodegeneration. Trends Neurosci 39:235-45
Wang, Yubin; Lopez, Dulce; Davey, Pinakin Gunvant et al. (2016) Calpain-1 and calpain-2 play opposite roles in retinal ganglion cell degeneration induced by retinal ischemia/reperfusion injury. Neurobiol Dis 93:121-8
Chen, Yuncai; Molet, Jenny; Lauterborn, Julie C et al. (2016) Converging, Synergistic Actions of Multiple Stress Hormones Mediate Enduring Memory Impairments after Acute Simultaneous Stresses. J Neurosci 36:11295-11307
Wang, Yubin; Hersheson, Joshua; Lopez, Dulce et al. (2016) Defects in the CAPN1 Gene Result in Alterations in Cerebellar Development and Cerebellar Ataxia in Mice and Humans. Cell Rep 16:79-91
Lauterborn, Julie C; Palmer, Linda C; Jia, Yousheng et al. (2016) Chronic Ampakine Treatments Stimulate Dendritic Growth and Promote Learning in Middle-Aged Rats. J Neurosci 36:1636-46
Sun, Jiandong; Liu, Yan; Tran, Jennifer et al. (2016) mTORC1-S6K1 inhibition or mTORC2 activation improves hippocampal synaptic plasticity and learning in Angelman syndrome mice. Cell Mol Life Sci 73:4303-4314
Liu, Yan; Wang, Yubin; Zhu, Guoqi et al. (2016) A calpain-2 selective inhibitor enhances learning & memory by prolonging ERK activation. Neuropharmacology 105:471-7
Wang, Weisheng; Kantorovich, Svetlana; Babayan, Alex H et al. (2016) Estrogen's Effects on Excitatory Synaptic Transmission Entail Integrin and TrkB Transactivation and Depend Upon β1-integrin function. Neuropsychopharmacology 41:2723-32
Liu, Yan; Sun, Jiandong; Wang, Yubin et al. (2016) Deleting both PHLPP1 and CANP1 rescues impairments in long-term potentiation and learning in both single knockout mice. Learn Mem 23:399-404

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