Mouse Physiology Core (Chamberlain,JS, P.I.) The Mouse Physiology Core provides advice and trainingon basic aspects of musclephysiologyand histopathological methodologies. This is a small, highly focused Core designed simply to provideexpert assistance to members of this PPG on a limited number of muscle physiology assays that are widley used in our studies of muscle disorders. It is not intended to be a research and devlopment core, and the limitedscope is in line with a very modest budget request. Experimental aspects of the Core are focused on providingassistance with viral vector injections into experimentalanimals, assisting with treadmillassays and performingmeasurements of contractile properties of key muscles, which constitutes a major hallmark of dystrophic pathophysiology and a sensitive index of overallmuscle health and function.

Public Health Relevance

There are few laboratories in the United States able to perform detailed analysis of muscle mechanical properties in conjunction with viral vector delivery to dystrophic animals. Our group has developed state-of- the-art protocols to enable detailed study of the functional effects of systemic gene delivery, and the Core allows non-specialists, such as moleuclar biologists, access to these informative, physiological analyses.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS046788-10
Application #
8447011
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
10
Fiscal Year
2013
Total Cost
$39,504
Indirect Cost
$14,180
Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
Parker, Maura H; Tapscott, Stephen J (2013) Expanding donor muscle-derived cells for transplantation. Curr Protoc Stem Cell Biol Chapter 2:Unit 2C.4
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Tai, Phillip W L; Smith, Catherine L; Angello, John C et al. (2012) Analysis of fiber-type differences in reporter gene expression of ?-gal transgenic muscle. Methods Mol Biol 798:445-59
Goncalves, Manuel A F V; Janssen, Josephine M; Nguyen, Quynh G et al. (2011) Transcription factor rational design improves directed differentiation of human mesenchymal stem cells into skeletal myocytes. Mol Ther 19:1331-41
Suga, Tomohiro; Kimura, En; Morioka, Yuka et al. (2011) Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse. PLoS One 6:e16908
Banks, Glen B; Combs, Ariana C; Chamberlain, Jeffrey S (2010) Sequencing protocols to genotype mdx, mdx(4cv), and mdx(5cv) mice. Muscle Nerve 42:268-70
Kimura, En; Li, Sheng; Gregorevic, Paul et al. (2010) Dystrophin delivery to muscles of mdx mice using lentiviral vectors leads to myogenic progenitor targeting and stable gene expression. Mol Ther 18:206-13
Hall, John K; Banks, Glen B; Chamberlain, Jeffrey S et al. (2010) Prevention of muscle aging by myofiber-associated satellite cell transplantation. Sci Transl Med 2:57ra83
Himeda, Charis L; Ranish, Jeffrey A; Pearson, Richard C M et al. (2010) KLF3 regulates muscle-specific gene expression and synergizes with serum response factor on KLF binding sites. Mol Cell Biol 30:3430-43
Banks, Glen B; Chamberlain, Jeffrey S; Froehner, Stanley C (2009) Truncated dystrophins can influence neuromuscular synapse structure. Mol Cell Neurosci 40:433-41

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