All three projects proposed in the program project grant application will utilize the mouse behavioral core facilities including motor and cognitive testing. Motor testing is most often done as a counterpart to histopathology to assess brain damage after stroke, as well as effects of molecular pathway manipulation and putative therapeutic strategies. More recently it has become appreciated that stroke also induces cognitive deficits as well, yet cognitive testing is only occasionally used as a measure of functional outcome after experimental stroke. In contrast, cognitive output is a mainstay of assessment in traumatic brain injury (TBI) models. Most cognitive testing reported involves use of memory acquisition tests such as the Morris water maze, T-maze, novel object recognition, and others. Because each of the individual projects relies on behavioral testing as important experimental outputs, the sensitivity, validity, and reliability of behavioral testing is a central issue in the PPG overall. This is especially true when comparing and interpreting behavioral data across diverse injury models such as stroke and TBI. The goal of the behavioral core is to provide a central facility to each PI that will (1) assist in experimental design and selection of appropriate testing methodology to answer specific questions in individual brain injury models, (2) ensure a uniform non- varying testing environment to provide stable environmental and test conditions longitudinally across experiments, and (3) provide hands on assistance and technical training in motor and cognitive testing for technicians, postdoctoral fellows, graduate students, faculty and others interested in expanding the behavioral testing repertoire of their labs. The behavioral core will be equipped to perform motor (corner test, cylinder test, grid walk, and tape removal) and cognitive (Morris water maze. Y-maze, novel object recognition, and radial arm maze) testing using video camera recorders and Any Maze software. In addition to its availability for all of the Pis, the behaviroal core facility will be open for use by other NIH-funded investigators at MGH as time and space allow.

Public Health Relevance

The purpose of the PPG is to utilize and assess long term regenerative strategies to promote recovery after stroke and traumatic brain injury. Functional (motor and cognitive) outcome measures require robust testing methods that are consistent over time to ensure their reliability and validity. The Behavioral Core will provide equipment and standardized protocols to ensure validity of data across experiments.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
2P01NS055104-06A1
Application #
8663356
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
Shindo, Akihiro; Maki, Takakuni; Mandeville, Emiri T et al. (2016) Astrocyte-Derived Pentraxin 3 Supports Blood-Brain Barrier Integrity Under Acute Phase of Stroke. Stroke 47:1094-100
Chung, David Y; Oka, Fumiaki; Ayata, Cenk (2016) Spreading Depolarizations: A Therapeutic Target Against Delayed Cerebral Ischemia After Subarachnoid Hemorrhage. J Clin Neurophysiol 33:196-202
Liang, Anna C; Mandeville, Emiri T; Maki, Takakuni et al. (2016) Effects of Aging on Neural Stem/Progenitor Cells and Oligodendrocyte Precursor Cells After Focal Cerebral Ischemia in Spontaneously Hypertensive Rats. Cell Transplant 25:705-14
Seidel, Jessica L; Escartin, Carole; Ayata, Cenk et al. (2016) Multifaceted roles for astrocytes in spreading depolarization: A target for limiting spreading depolarization in acute brain injury? Glia 64:5-20
Wu, Limin; Ramirez, Servio H; Andrews, Allison M et al. (2016) Neuregulin1-β decreases interleukin-1β-induced RhoA activation, myosin light chain phosphorylation, and endothelial hyperpermeability. J Neurochem 136:250-7
Xing, Changhong; Lo, Eng H (2016) Help-me signaling: Non-cell autonomous mechanisms of neuroprotection and neurorecovery. Prog Neurobiol :
Mandeville, Emiri T; Ayata, Cenk; Zheng, Yi et al. (2016) Translational MR Neuroimaging of Stroke and Recovery. Transl Stroke Res :
Egawa, Naohiro; Lok, Josephine; Washida, Kazuo et al. (2016) Mechanisms of Axonal Damage and Repair after Central Nervous System Injury. Transl Stroke Res :
Arai, Ken (2016) Stroke Literature Synopses: Basic Science. Stroke 47:e250-e251
Arai, Ken (2016) Stroke Literature Synopses: Basic Science. Stroke 47:e40-1

Showing the most recent 10 out of 202 publications