Core B is essential to support the microscopic imaging, quantitative analysis, and peripheral nerve injury model used in this PPG. The Core is used extensively by all three projects and contributes directly to multiple goals of these projects. The scope and resources of Core B have been significantly expanded by addition or update of new instrumentation (e.g. an AMT digital camera for the electron microscope, funded by an Administrative Supplement to the PPG) to meet the needs of the PPG projects. A significant addition is the provision of expertise and technical help to provide a single model of peripheral nerve injury in rodents that is used by all 3 projects. This eliminates variability between projects in surgical technique and microinjection of tracers in specific muscles or nerves, and facilitates direct comparison of data between projects to aid in interpretation. The Core facility is directed by a highly experienced investigator and research administrator, assisted by three highly trained and qualified research associates. The Core provides all necessary training to members of the PPG groups for authorized access and use of Core instruments and facilities;PPG members have priority access to the facility. Services include training and guidance for use of instruments and analytical techniques, assistance with design and/or interpretation of experiments, hands-on performance of tissue processing and imaging as required, electron microscopy processing and imaging, and microsurgery and tracer injection. Significant resources include 3 confocal microscopes (one is a multiphoton instrument with electrophysiology setup), electron microscope suite, Neurolucida system, fluorescence dissecting microscope, cryostats, vibratomes and ultramicrotomes, cryosubstitution system, a fully equipped histology laboratory, access to a state-of-the-art suite for microsurgery in rodents, and four off-line workstations for image processing and data analysis. Data is securely stored, and each PPG PI has secure access to a PPG shared data Cloud (1.0 terabyte) where they can access their own and each other's data and communicate seamlessly with the data acquisition instruments in the Core.

Public Health Relevance

This shared core facility provides instruments and technical resources for experiments and analysis that cannot be provided by the individual laboratories. The core provides consistency in experimental and analytical approaches, thus reducing variability and aiding interpretation of results, and promotes close collaborations in the effort to understand and improve recovery from the effects of peripheral nerve injury.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Program Projects (P01)
Project #
Application #
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Wright State University
United States
Zip Code
Obeidat, Ahmed Z; Nardelli, Paul; Powers, Randall K et al. (2014) Modulation of motoneuron firing by recurrent inhibition in the adult rat in vivo. J Neurophysiol 112:2302-15
Rotterman, Travis M; Nardelli, Paul; Cope, Timothy C et al. (2014) Normal distribution of VGLUT1 synapses on spinal motoneuron dendrites and their reorganization after nerve injury. J Neurosci 34:3475-92
Deardorff, Adam S; Romer, Shannon H; Sonner, Patrick M et al. (2014) Swimming against the tide: investigations of the C-bouton synapse. Front Neural Circuits 8:106
Romer, Shannon H; Dominguez, Kathleen M; Gelpi, Marc W et al. (2014) Redistribution of Kv2.1 ion channels on spinal motoneurons following peripheral nerve injury. Brain Res 1547:1-15
Zhang, Jingming; Lanuza, Guillermo M; Britz, Olivier et al. (2014) V1 and v2b interneurons secure the alternating flexor-extensor motor activity mice require for limbed locomotion. Neuron 82:138-50
Koesters, Andrew; Engisch, Kathrin L; Rich, Mark M (2014) Decreased cardiac excitability secondary to reduction of sodium current may be a significant contributor to reduced contractility in a rat model of sepsis. Crit Care 18:R54
Deardorff, Adam S; Romer, Shannon H; Deng, Zhihui et al. (2013) Expression of postsynaptic Ca2+-activated K+ (SK) channels at C-bouton synapses in mammalian lumbar -motoneurons. J Physiol 591:875-97
Nardelli, Paul; Khan, Jaffar; Powers, Randall et al. (2013) Reduced motoneuron excitability in a rat model of sepsis. J Neurophysiol 109:1775-81
Wang, Xueyong; Wang, Qingbo; Yang, Shuzhang et al. (2011) Impaired activity-dependent plasticity of quantal amplitude at the neuromuscular junction of Rab3A deletion and Rab3A earlybird mutant mice. J Neurosci 31:3580-8
Bullinger, Katie L; Nardelli, Paul; Pinter, Martin J et al. (2011) Permanent central synaptic disconnection of proprioceptors after nerve injury and regeneration. II. Loss of functional connectivity with motoneurons. J Neurophysiol 106:2471-85

Showing the most recent 10 out of 20 publications