The Animal Models, Histology and Tissue Bank Core will maintain availability and assistance in the induction of all murine models of experimental autoimmune encephalomyelitis (EAE) including monophasic and relapsing-remitting models induced by both active immunization and adoptive transfer of encephalitogenic T cells. Towards this latter goal, Core B will generate and maintain encephalitogenic and control T cell lines. Core B will also assist in all outcome measurements, histological analyses and immunohistochemical approaches. To support PPG investigators? exploration of alternative approaches and establish future goals for all Projects, Core B will also establish a murine tissue bank for screening purposes. Because the overall goal of all animals work is to translate findings to human tissues and, ultimately, patients, Core B will establish and maintain a human CNS tissue bank with histopathologic analyses from control and MS patients plus banked peripheral blood mononuclear cells, sera and cerebrospinal fluid. These samples will be utilized by all Projects to validate findings in murine models and to further investigate findings in murine systems using human tissues. We recently hired a dedicated technician to process, catalogue and coordinate the evaluation of CNS specimens for inflammation, demyelination, axonal injury and remyelination. In summary, the function of Core B is to provide technical support for a variety of in vivo experimentation using murine MS models and to facilitate studies for the translation of findings to MS patients via use of human specimens.

Public Health Relevance

Core B is responsible for providing technical assistance for all PPG investigators utilizing animal models of MS or evaluating human specimens. The extent of this support ranges from outcome measurements and histological analyses to live animal imaging within the central nervous system. Core B therefore provides major support for bench-to-bedside translation of findings.

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National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
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Washington University
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Cross, Anne H; Song, Sheng-Kwei (2016) "A new imaging modality to non-invasively assess multiple sclerosis pathology". J Neuroimmunol :
Kim, Joong Hee; Song, Sheng-Kwei; Haldar, Justin P (2016) Signal-to-noise ratio-enhancing joint reconstruction for improved diffusion imaging of mouse spinal cord white matter injury. Magn Reson Med 75:852-8
Salimi, Hamid; Cain, Matthew D; Klein, Robyn S (2016) Encephalitic Arboviruses: Emergence, Clinical Presentation, and Neuropathogenesis. Neurotherapeutics 13:514-34
Yue, Xuyi; Jin, Hongjun; Liu, Hui et al. (2015) A potent and selective C-11 labeled PET tracer for imaging sphingosine-1-phosphate receptor 2 in the CNS demonstrates sexually dimorphic expression. Org Biomol Chem 13:7928-39
Cantoni, Claudia; Bollman, Bryan; Licastro, Danilo et al. (2015) TREM2 regulates microglial cell activation in response to demyelination in vivo. Acta Neuropathol 129:429-47
Wang, Yong; Sun, Peng; Wang, Qing et al. (2015) Differentiation and quantification of inflammation, demyelination and axon injury or loss in multiple sclerosis. Brain 138:1223-38
Daniels, Brian P; Klein, Robyn S (2015) Knocking on Closed Doors: Host Interferons Dynamically Regulate Blood-Brain Barrier Function during Viral Infections of the Central Nervous System. PLoS Pathog 11:e1005096
Tu, Tsang-Wei; Budde, Matthew D; Xie, Mingqiang et al. (2014) Phase-aligned multiple spin-echo averaging: a simple way to improve signal-to-noise ratio of in vivo mouse spinal cord diffusion tensor image. Magn Reson Imaging 32:1335-43
Cruz-Orengo, Lillian; Daniels, Brian P; Dorsey, Denise et al. (2014) Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility. J Clin Invest 124:2571-84
Lin, Tsen-Hsuan; Chiang, Chia-Wen; Trinkaus, Kathryn et al. (2014) Manganese-enhanced MRI (MEMRI) via topical loading of Mn(2+) significantly impairs mouse visual acuity: a comparison with intravitreal injection. NMR Biomed 27:390-8

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