The Animal Models, Histology and Tissue Bank Core will maintain availability and assistance in the induction of all murine models of experimental autoimmune encephalomyelitis (EAE) including monophasic and relapsing-remitting models induced by both active immunization and adoptive transfer of encephalitogenic T cells. Towards this latter goal, Core B will generate and maintain encephalitogenic and control T cell lines. Core B will also assist in all outcome measurements, histological analyses and immunohistochemical approaches. To support PPG investigators? exploration of alternative approaches and establish future goals for all Projects, Core B will also establish a murine tissue bank for screening purposes. Because the overall goal of all animals work is to translate findings to human tissues and, ultimately, patients, Core B will establish and maintain a human CNS tissue bank with histopathologic analyses from control and MS patients plus banked peripheral blood mononuclear cells, sera and cerebrospinal fluid. These samples will be utilized by all Projects to validate findings in murine models and to further investigate findings in murine systems using human tissues. We recently hired a dedicated technician to process, catalogue and coordinate the evaluation of CNS specimens for inflammation, demyelination, axonal injury and remyelination. In summary, the function of Core B is to provide technical support for a variety of in vivo experimentation using murine MS models and to facilitate studies for the translation of findings to MS patients via use of human specimens.

Public Health Relevance

Core B is responsible for providing technical assistance for all PPG investigators utilizing animal models of MS or evaluating human specimens. The extent of this support ranges from outcome measurements and histological analyses to live animal imaging within the central nervous system. Core B therefore provides major support for bench-to-bedside translation of findings.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Program Projects (P01)
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National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
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Washington University
Saint Louis
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Agner, Shannon C; Klein, Robyn S (2018) Viruses have multiple paths to central nervous system pathology. Curr Opin Neurol 31:313-317
Adusumilli, Gautam; Trinkaus, Kathryn; Sun, Peng et al. (2018) Intensity ratio to improve black hole assessment in multiple sclerosis. Mult Scler Relat Disord 19:140-147
Spees, William M; Lin, Tsen-Hsuan; Sun, Peng et al. (2018) MRI-based assessment of function and dysfunction in myelinated axons. Proc Natl Acad Sci U S A 115:E10225-E10234
Zhan, Jie; Lin, Tsen-Hsuan; Libbey, Jane E et al. (2018) Diffusion Basis Spectrum and Diffusion Tensor Imaging Detect Hippocampal Inflammation and Dendritic Injury in a Virus-Induced Mouse Model of Epilepsy. Front Neurosci 12:77
Klein, Robyn S; Garber, Charise; Howard, Nicole (2017) Infectious immunity in the central nervous system and brain function. Nat Immunol 18:132-141
Lin, Tsen-Hsuan; Chiang, Chia-Wen; Perez-Torres, Carlos J et al. (2017) Diffusion MRI quantifies early axonal loss in the presence of nerve swelling. J Neuroinflammation 14:78
Cross, Anne H; Song, Sheng-Kwei (2017) ""A new imaging modality to non-invasively assess multiple sclerosis pathology"". J Neuroimmunol 304:81-85
Klein, Robyn S; Hunter, Christopher A (2017) Protective and Pathological Immunity during Central Nervous System Infections. Immunity 46:891-909
Hou, Jianghui; Baker, Lane A; Zhou, Lushan et al. (2016) Viral interactions with the blood-brain barrier: old dog, new tricks. Tissue Barriers 4:e1142492
Salimi, Hamid; Cain, Matthew D; Klein, Robyn S (2016) Encephalitic Arboviruses: Emergence, Clinical Presentation, and Neuropathogenesis. Neurotherapeutics 13:514-34

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