The goal of this program is to investigate the roles of conventional and 'unconventional'mitochondrial proteins in the pathogenesis of Parkinson's disease (PD). The major roles of the Molecular Core are to (i) provide the genes or gene products that are required in the individual projects, and (2) alter the expression of proteins by either knockdown (shRNA) or overexpression of their genes to facilitate the investigation of the pathogenesis of PD proposed in the individual projects. Services provided by the Molecular Core include: cDNA cloning, design and production of constructs for gene overexpression or gene silencing, assistance with generation of transient and stable transfections, production of viral vectors for in vivo gene transfer, and generation of fusion proteins containing protein transduction domain (TAT) for efficient protein transduction in in vitro and in vivo. These services will be critical for successful completion of each of the individual projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS059806-05
Application #
8505555
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
5
Fiscal Year
2013
Total Cost
$170,324
Indirect Cost
$57,899
Name
University of Pittsburgh
Department
Type
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
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Tapias, Victor; Greenamyre, J Timothy (2014) A rapid and sensitive automated image-based approach for in vitro and in vivo characterization of cell morphology and quantification of cell number and neurite architecture. Curr Protoc Cytom 68:12.33.1-12.33.22
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Tapias, Victor; Greenamyre, J Timothy; Watkins, Simon C (2013) Automated imaging system for fast quantitation of neurons, cell morphology and neurite morphometry in vivo and in vitro. Neurobiol Dis 54:158-68
Sanders, Laurie H; Greenamyre, J Timothy (2013) Oxidative damage to macromolecules in human Parkinson disease and the rotenone model. Free Radic Biol Med 62:111-20

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