The goal of this program is to investigate the roles of conventional and 'unconventional'mitochondrial proteins in the pathogenesis of Parkinson's disease (PD). The major roles of the Molecular Core are to (i) provide the genes or gene products that are required in the individual projects, and (2) alter the expression of proteins by either knockdown (shRNA) or overexpression of their genes to facilitate the investigation of the pathogenesis of PD proposed in the individual projects. Services provided by the Molecular Core include: cDNA cloning, design and production of constructs for gene overexpression or gene silencing, assistance with generation of transient and stable transfections, production of viral vectors for in vivo gene transfer, and generation of fusion proteins containing protein transduction domain (TAT) for efficient protein transduction in in vitro and in vivo. These services will be critical for successful completion of each of the individual projects.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Program Projects (P01)
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National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
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University of Pittsburgh
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An, Chengrui; Shi, Yejie; Li, Peiying et al. (2014) Molecular dialogs between the ischemic brain and the peripheral immune system: dualistic roles in injury and repair. Prog Neurobiol 115:6-24
Tapias, Victor; Greenamyre, J Timothy (2014) A rapid and sensitive automated image-based approach for in vitro and in vivo characterization of cell morphology and quantification of cell number and neurite architecture. Curr Protoc Cytom 68:12.33.1-12.33.22
Mohammadyani, Dariush; Tyurin, Vladimir A; O'Brien, Matthew et al. (2014) Molecular speciation and dynamics of oxidized triacylglycerols in lipid droplets: Mass spectrometry and coarse-grained simulations. Free Radic Biol Med 76:53-60
Hu, Xiaoming; Liou, Anthony K F; Leak, Rehana K et al. (2014) Neurobiology of microglial action in CNS injuries: receptor-mediated signaling mechanisms and functional roles. Prog Neurobiol 119-120:60-84
Tapias, Victor; Cannon, Jason R; Greenamyre, J Timothy (2014) Pomegranate juice exacerbates oxidative stress and nigrostriatal degeneration in Parkinson's disease. Neurobiol Aging 35:1162-76
Sanders, Laurie H; McCoy, Jennifer; Hu, Xiaoping et al. (2014) Mitochondrial DNA damage: molecular marker of vulnerable nigral neurons in Parkinson's disease. Neurobiol Dis 70:214-23
Sanders, Laurie H; Laganiere, Josee; Cooper, Oliver et al. (2014) LRRK2 mutations cause mitochondrial DNA damage in iPSC-derived neural cells from Parkinson's disease patients: reversal by gene correction. Neurobiol Dis 62:381-6
Wang, Jiayin; Shi, Yejie; Zhang, Lili et al. (2014) Omega-3 polyunsaturated fatty acids enhance cerebral angiogenesis and provide long-term protection after stroke. Neurobiol Dis 68:91-103
Tapias, Victor; Greenamyre, J Timothy; Watkins, Simon C (2013) Automated imaging system for fast quantitation of neurons, cell morphology and neurite morphometry in vivo and in vitro. Neurobiol Dis 54:158-68
Sanders, Laurie H; Greenamyre, J Timothy (2013) Oxidative damage to macromolecules in human Parkinson disease and the rotenone model. Free Radic Biol Med 62:111-20

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