A major cause of chronic disability in survivors of premature birth is the development of periventricular leukolomalacia and ventriculomegaly, with subsequent cerebral palsy and cognitive impairment. The primary pathological hallmark of this condition is white matter (WM) atrophy resulfing from the loss of myelin and oligodendrocytes. The cellular pathophysiology underiying the altered development of WM in premature children is complex and not fully understood. WM glia, particularly progenitors for oligodendrocytes and astrocytes, is susceptible to hypoxic injury. Oligodendrocytes and astrocytes play essenfial roles in WM integrity and funcfion, therefore a thorough understanding of the cellular mechanisms that, lead to glial maldevelopment and damage after hypoxia (HX) is necessary. The identificafion of molecular mechanisms that are crucial to regulate glial regeneration will enable: i) manipulafion of glia and progenitor cells to preserve WM integrity, and ii) enhanced regeneration and recovery from postnatal HXinduced injury. We investigated the role of EGFR signaling in developmental myelination after HX in a CNP-hEGFR mouse, in which all oligodendrocyte precursor cells overexpress the hEGFR. Our preliminary analysis shows that enhanced EGFR activity protects oligodendrocytes from HX-induced damage and promotes a recovery response that involves acfivation of oligodendrocyte progenitor cells (OPCs). Based on these findings, we are planning to invesfigate the role of EGFR signaling in WM development after hypoxic damage. First, we will define and compare development of WM glia and myelinafion in WT and CNP-hEGFR mice after HX. A comparafive cellular and molecular analysis of glial progenitors in the subventricular zone will also be performed. Second, we will determine the extent of functional myelinafion in WT and CNP-hEGFR mice after HX by electrophysiological analysis. Diffusion tensor imaging (DTI) and behavioral evaluation will also be used to determine the extent of structural and functional recovery. Finally, we will assess whether maintaining hypoxic mice in an enriched environment improves WM glia recovery and myelinafion, and whether enhanced EGFR signaling adds further to the beneficial effects of enriched environment. Together, these studies will not only shed light on crucial cellular and molecular mechanisms of WM injury and recovery, but might also lead to the development of new therapeutic approaches aimed at lessening the long-term neurological sequela of premature birth.

Public Health Relevance

The goal of Project 3 is to understand whether chronic sublethal hypoxia impairs the survival and the maturation of oligodendrocytes in the developing white matter, and whether this can be overcome by enhancing oligodendrogenesis from endogenous progenitor cells. Understanding the cellular signals that promote oligodendrocyte generafion and survival after injury will allow developing new means of therapeufic intervenfion to the neurobehavioral sequelae of preterm birth.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS062686-04
Application #
8375348
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
4
Fiscal Year
2012
Total Cost
$245,271
Indirect Cost
Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
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