This critically important core provides routine and consistent analysis of spleen cells, cervical lymph node cells, and resident as well as inflammatory cells derived from the CNS. As noted in the individual projects, precise analysis of the cells infiltrating the CNS during infection, as well as the resident CNS cells (oligodendroglia, astrocytes and microglia) are key components of the overall program. This includes measurement of surface markers, immune modulatory molecules, and cytokines expressed during immune activation by both resident and infiltrating cell types. Precise identification and separation of cell types is required for both acquisition and analysis and cell purification by sorting. This Core will provide quality control for efficacy and purity of various cell sorting strategies required for each project. This Core will furthermore interact with the Animal Core (Core B) by confirming reconstitution of kjone marrow chimeras and genetic phenotypes in the breeding colonies. The major functions are thus to: 1) provide routine acquisition and analysis of cell composition and their phenotypic changes in various tissues;2) purify cells from various mouse tissues for gene profiling and immunological assays in vitro;3) verify the efficacy of bone marrow reconstitution;4) analyze PBMC for genotyping;and 5) provide quality control for cell separation and purity. Performance of all these tasks fulfills essential functions for all three projects. Core B is critical to accomplishing the overall scientific goals of the entire project.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS064932-04
Application #
8382534
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
4
Fiscal Year
2012
Total Cost
$195,310
Indirect Cost
$84,686
Name
Cleveland Clinic Lerner
Department
Type
DUNS #
135781701
City
Cleveland
State
OH
Country
United States
Zip Code
44195
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Phares, Timothy W; DiSano, Krista D; Hinton, David R et al. (2013) IL-21 optimizes T cell and humoral responses in the central nervous system during viral encephalitis. J Neuroimmunol 263:43-54
de Aquino, Maria Teresa P; Puntambekar, Shweta S; Savarin, Carine et al. (2013) Role of CD25(+) CD4(+) T cells in acute and persistent coronavirus infection of the central nervous system. Virology 447:112-20
Phares, Timothy W; Stohlman, Stephen A; Hinton, David R et al. (2013) Astrocyte-derived CXCL10 drives accumulation of antibody-secreting cells in the central nervous system during viral encephalomyelitis. J Virol 87:3382-92
Phares, Timothy W; Stohlman, Stephen A; Hwang, Mihyun et al. (2012) CD4 T cells promote CD8 T cell immunity at the priming and effector site during viral encephalitis. J Virol 86:2416-27

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