The Neuropathology Core (Core D) functions as an integral component of each of the Projects providing histopathologic services and Neuropathologic expertise. The Core PI (David R Hinton MD) and Core Colnvestigator (Roscoe Atkinson MD) are both board certified Neuropathologists with extensive experience in the evaluation of murine central nervous system (CNS) tissues. Support provided to each of the projects includes: 1) participation in development of experimental design for animal experiments;2) tissue processing;3) tissue sectioning (frozen sections and paraffin-embedded sections);4) histochemical and immunohistochemical staining of tissue sections;5) neuropathologic interpretation of tissue sections (by light microscopy or confocal microscopy);6) documentation ofthe pathology by digital microscopy;7) preparation of figures for manuscripts;8) storage and retrieval of stained slides for each of the projects. Expert preparation and neuropathologic evaluation of tissues ensures high quality, unbiased assessment of CNS tissues for pathologic alterations including inflammation, demyelination and viral infection.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS064932-04
Application #
8382537
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
4
Fiscal Year
2012
Total Cost
$180,607
Indirect Cost
Name
Cleveland Clinic Lerner
Department
Type
DUNS #
135781701
City
Cleveland
State
OH
Country
United States
Zip Code
44195
Butchi, Niranjan; Kapil, Parul; Puntambekar, Shweta et al. (2015) Myd88 Initiates Early Innate Immune Responses and Promotes CD4 T Cells during Coronavirus Encephalomyelitis. J Virol 89:9299-312
Butchi, Niranjan B; Hinton, David R; Stohlman, Stephen A et al. (2014) Ifit2 deficiency results in uncontrolled neurotropic coronavirus replication and enhanced encephalitis via impaired alpha/beta interferon induction in macrophages. J Virol 88:1051-64
de Aquino, Maria Teresa P; Kapil, Parul; Hinton, David R et al. (2014) IL-27 limits central nervous system viral clearance by promoting IL-10 and enhances demyelination. J Immunol 193:285-94
Hwang, Mihyun; Phares, Timothy W; Hinton, David R et al. (2014) Distinct CD4 T cell effects on primary versus recall CD8 T cell responses during viral encephalomyelitis. Immunology :
Kapil, Parul; Stohlman, Stephen A; Hinton, David R et al. (2014) PKR mediated regulation of inflammation and IL-10 during viral encephalomyelitis. J Neuroimmunol 270:1-12
Savarin, Carine; Bergmann, Cornelia C; Hinton, David R et al. (2013) MMP-independent role of TIMP-1 at the blood brain barrier during viral encephalomyelitis. ASN Neuro 5:e00127
Phares, Timothy W; DiSano, Krista D; Hinton, David R et al. (2013) IL-21 optimizes T cell and humoral responses in the central nervous system during viral encephalitis. J Neuroimmunol 263:43-54
de Aquino, Maria Teresa P; Puntambekar, Shweta S; Savarin, Carine et al. (2013) Role of CD25(+) CD4(+) T cells in acute and persistent coronavirus infection of the central nervous system. Virology 447:112-20
Phares, Timothy W; Stohlman, Stephen A; Hinton, David R et al. (2013) Astrocyte-derived CXCL10 drives accumulation of antibody-secreting cells in the central nervous system during viral encephalomyelitis. J Virol 87:3382-92
Phares, Timothy W; Stohlman, Stephen A; Hwang, Mihyun et al. (2012) CD4 T cells promote CD8 T cell immunity at the priming and effector site during viral encephalitis. J Virol 86:2416-27

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