The Neuropathology Core (Core D) functions as an integral component of each of the Projects providing histopathologic services and Neuropathologic expertise. The Core PI (David R Hinton MD) and Core Colnvestigator (Roscoe Atkinson MD) are both board certified Neuropathologists with extensive experience in the evaluation of murine central nervous system (CNS) tissues. Support provided to each of the projects includes: 1) participation in development of experimental design for animal experiments;2) tissue processing;3) tissue sectioning (frozen sections and paraffin-embedded sections);4) histochemical and immunohistochemical staining of tissue sections;5) neuropathologic interpretation of tissue sections (by light microscopy or confocal microscopy);6) documentation ofthe pathology by digital microscopy;7) preparation of figures for manuscripts;8) storage and retrieval of stained slides for each of the projects. Expert preparation and neuropathologic evaluation of tissues ensures high quality, unbiased assessment of CNS tissues for pathologic alterations including inflammation, demyelination and viral infection.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Program Projects (P01)
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National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
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Cleveland Clinic Lerner
United States
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Butchi, Niranjan B; Hinton, David R; Stohlman, Stephen A et al. (2014) Ifit2 deficiency results in uncontrolled neurotropic coronavirus replication and enhanced encephalitis via impaired alpha/beta interferon induction in macrophages. J Virol 88:1051-64
de Aquino, Maria Teresa P; Kapil, Parul; Hinton, David R et al. (2014) IL-27 limits central nervous system viral clearance by promoting IL-10 and enhances demyelination. J Immunol 193:285-94
Kapil, Parul; Stohlman, Stephen A; Hinton, David R et al. (2014) PKR mediated regulation of inflammation and IL-10 during viral encephalomyelitis. J Neuroimmunol 270:1-12
Phares, Timothy W; DiSano, Krista D; Hinton, David R et al. (2013) IL-21 optimizes T cell and humoral responses in the central nervous system during viral encephalitis. J Neuroimmunol 263:43-54
Phares, Timothy W; Stohlman, Stephen A; Hinton, David R et al. (2013) Astrocyte-derived CXCL10 drives accumulation of antibody-secreting cells in the central nervous system during viral encephalomyelitis. J Virol 87:3382-92
Phares, Timothy W; Stohlman, Stephen A; Hwang, Mihyun et al. (2012) CD4 T cells promote CD8 T cell immunity at the priming and effector site during viral encephalitis. J Virol 86:2416-27
Puntambekar, Shweta S; Bergmann, Cornelia C; Savarin, Carine et al. (2011) Shifting hierarchies of interleukin-10-producing T cell populations in the central nervous system during acute and persistent viral encephalomyelitis. J Virol 85:6702-13
Savarin, Carine; Stohlman, Stephen A; Rietsch, Anna M et al. (2011) MMP9 deficiency does not decrease blood-brain barrier disruption, but increases astrocyte MMP3 expression during viral encephalomyelitis. Glia 59:1770-81
Phares, Timothy W; Stohlman, Stephen A; Hinton, David R et al. (2010) Enhanced antiviral T cell function in the absence of B7-H1 is insufficient to prevent persistence but exacerbates axonal bystander damage during viral encephalomyelitis. J Immunol 185:5607-18