The objective of the Morphology Core is to support the Center projects with high-quality and specialized morphological services while achieving net cost effectiveness. The services to be offered are classified into four categories: 1. Standard staining of sections following paraffin embedding of tissues (e.g. hematoxylin and eosin, Sirius red or Masson trichrome for collagen);2. Immunohistochemical staining for fixed or frozen sections using different primary antibodies and probes (e.g. CDc37, Hsp 90, ROS species, KLF6, CYP2E1, among others)) in alcoholic liver injury;3. image and morphometric analysis to provide standardized quantitative data. 4. tissue microarray. In addition, the Core can provide electron microscopic examination of tissue to identify ultrastructural alterations of cells. Standard and special staining procedures are carried out on samples after preparation of frozen sections, fixation and embedding, cutting, and mounting on slides. Morphometric/image analysis is performed for quantification of histologic assessment, immunostained proteins or cells by digital imaging analysis using a digital camera, Nikon Coolscope, and captured by a Samba imaging system equipped with a Compaq center computer embedded with a Matrox IM-640 imaging board connected to input components including a Zeiss Axioskop microscope and a Hamamatsu 3CCD camera. Quality control is maintained at the Core site, the Department of Pathology at The Mount Sinai Medical Center, which is inspected annually and certified by the College of American Pathology.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Exploratory Grants (P20)
Project #
5P20AA017067-05
Application #
8380264
Study Section
Special Emphasis Panel (ZAA1-BB)
Project Start
Project End
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
5
Fiscal Year
2012
Total Cost
$29,902
Indirect Cost
$12,259
Name
Icahn School of Medicine at Mount Sinai
Department
Type
DUNS #
078861598
City
New York
State
NY
Country
United States
Zip Code
10029
Renault, Thibaud T; Floros, Konstantinos V; Elkholi, Rana et al. (2015) Mitochondrial shape governs BAX-induced membrane permeabilization and apoptosis. Mol Cell 57:69-82
Kocabayoglu, Peri; Lade, Abigale; Lee, Youngmin A et al. (2015) β-PDGF receptor expressed by hepatic stellate cells regulates fibrosis in murine liver injury, but not carcinogenesis. J Hepatol 63:141-7
Kocabayoglu, Peri; Zhang, David Y; Kojima, Kensuke et al. (2015) Induction and contribution of beta platelet-derived growth factor signalling by hepatic stellate cells to liver regeneration after partial hepatectomy in mice. Liver Int :
Hasegawa, Daisuke; Calvo, Veronica; Avivar-Valderas, Alvaro et al. (2015) Epithelial Xbp1 is required for cellular proliferation and differentiation during mammary gland development. Mol Cell Biol 35:1543-56
Lade, Abigale; Noon, Luke A; Friedman, Scott L (2014) Contributions of metabolic dysregulation and inflammation to nonalcoholic steatohepatitis, hepatic fibrosis, and cancer. Curr Opin Oncol 26:100-7
Renault, Thibaud T; Elkholi, Rana; Bharti, Archana et al. (2014) B cell lymphoma-2 (BCL-2) homology domain 3 (BH3) mimetics demonstrate differential activities dependent upon the functional repertoire of pro- and anti-apoptotic BCL-2 family proteins. J Biol Chem 289:26481-91
Vacaru, Ana M; Unlu, Gokhan; Spitzner, Marie et al. (2014) In vivo cell biology in zebrafish - providing insights into vertebrate development and disease. J Cell Sci 127:485-95
Arriazu, Elena; Ruiz de Galarreta, Marina; Cubero, Francisco Javier et al. (2014) Extracellular matrix and liver disease. Antioxid Redox Signal 21:1078-97
Ge, Xiaodong; Antoine, Daniel J; Lu, Yongke et al. (2014) High mobility group box-1 (HMGB1) participates in the pathogenesis of alcoholic liver disease (ALD). J Biol Chem 289:22672-91
Howarth, Deanna L; Lindtner, Claudia; Vacaru, Ana M et al. (2014) Activating transcription factor 6 is necessary and sufficient for alcoholic fatty liver disease in zebrafish. PLoS Genet 10:e1004335

Showing the most recent 10 out of 82 publications