The Vanderbilt In Vivo Imaging Center (VIVID) will focus on a new optical and X-ray imaging modalities that have not yet been applied to cancer surveillance and management. Recent findings showing efficacy of anti-angiogenic approaches for cancer therapy emphasize the need for imaging modalities capable of evaluating tumor vascularity. Advances in laser scanning microscopy and fiber optics now permit true in vivo imaging, with resolution on the order of 100 nm. While application of optical methods may be minimally invasive, depending upon tissue sites, such procedures will deliver unprecedented speed and clarity. Furthermore, development of monochromatic X-ray imaging will provide resolutions below 30 nm. Development projects will be focused around the role of angiogenesis in tumor development and metastasis. The convergence of Vanderbilt expertise in imaging methodologies with interest of Vanderbilt-Ingram Cancer Center investigators provides a unique opportunity. Ability to image molecules, such as VEGF, MMP's and COX-2, in tumors at diagnosis will permit rational design of therapies tailored to the individual tumor and patient. Organizationally, core labs will be funded through direct collaborations with individual development projects, to maintain the focus on cancer diagnosis and treatment. Five core labs will be established based around existing facilities: 1) a mouse model core that will provide established mouse cancer models, as well as GFP transgenic and knock-in mice, 2) an in vitro imaging core that will support spectroscopy and microscopy, 3) an in vivo optical imaging core, that will use fiber-optic based and direct illumination modalities, 4) a monochromatic x-ray imaging core for in vivo morphological and molecular imaging, and finally 5) an MRI/Ultrasound/CT core that will be used to compare new methods with these accepted gold standards of in vivo imaging. These technologies span the range from cuvette to human subject. Collaborations between projects and cores promise to validate current hypotheses about tumor vascularization, and to rapidly apply these finding to clinical cancer diagnosis and therapy.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Exploratory Grants (P20)
Project #
5P20CA086283-02
Application #
6377892
Study Section
Special Emphasis Panel (ZCA1-SRRB-3 (J2))
Project Start
2000-06-01
Project End
2003-05-31
Budget Start
2001-06-01
Budget End
2002-05-31
Support Year
2
Fiscal Year
2001
Total Cost
$400,000
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Physiology
Type
Schools of Medicine
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Schietinger, Andrea; Arina, Ainhoa; Liu, Rebecca B et al. (2013) Longitudinal confocal microscopy imaging of solid tumor destruction following adoptive T cell transfer. Oncoimmunology 2:e26677
Uddin, Md Jashim; Crews, Brenda C; Blobaum, Anna L et al. (2010) Selective visualization of cyclooxygenase-2 in inflammation and cancer by targeted fluorescent imaging agents. Cancer Res 70:3618-27
Lieber, Chad A; Majumder, Shovan K; Billheimer, Dean et al. (2008) Raman microspectroscopy for skin cancer detection in vitro. J Biomed Opt 13:024013
Piston, David W; Rizzo, Mark A (2008) FRET by fluorescence polarization microscopy. Methods Cell Biol 85:415-30
Jansen, E Duco; Pickett, Patrick M; Mackanos, Mark A et al. (2006) Effect of optical tissue clearing on spatial resolution and sensitivity of bioluminescence imaging. J Biomed Opt 11:041119
Rizzo, Mark A; Springer, Gerald; Segawa, Katsuhisa et al. (2006) Optimization of pairings and detection conditions for measurement of FRET between cyan and yellow fluorescent proteins. Microsc Microanal 12:238-54
Kobayashi, Hanako; Lin, P Charles (2005) Angiopoietin/Tie2 signaling, tumor angiogenesis and inflammatory diseases. Front Biosci 10:666-74
Chen, Ying; Donnelly, Edwin; Kobayashi, Hanako et al. (2005) Gene therapy targeting the Tie2 function ameliorates collagen-induced arthritis and protects against bone destruction. Arthritis Rheum 52:1585-94
Nishishita, Toshishita; Lin, Pengnian Charles (2004) Angiopoietin 1, PDGF-B, and TGF-beta gene regulation in endothelial cell and smooth muscle cell interaction. J Cell Biochem 91:584-93
DeBusk, Laura M; Hallahan, Dennis E; Lin, Pengnian Charles (2004) Akt is a major angiogenic mediator downstream of the Ang1/Tie2 signaling pathway. Exp Cell Res 298:167-77

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