Abstract

The Proteomics Core will provide quantitative proteomics analysis for Pilot Projects l-lll using SILAM (pilots I, mouse model), SILAC (pilot II, cell culture model), and iTRAQ (pilot II, monocytes;pilot III, human platelet poor plasma) approaches. The Proteomics Core will also assist in preparing tissue and protein for these studies. Work will be integrated with the Bioinformatics &Biostatistics Core for data management, analysis and integration.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Exploratory Grants (P20)
Project #
5P20DA026149-03
Application #
8234086
Study Section
Special Emphasis Panel (ZDA1)
Project Start
Project End
2012-02-29
Budget Start
2011-03-01
Budget End
2012-02-29
Support Year
3
Fiscal Year
2011
Total Cost
$286,745
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Nika, Heinz; Angeletti, Ruth Hogue; Hawke, David H (2016) Phosphopeptide Enrichment by Covalent Chromatography After Solid Phase Derivatization of Protein Digests on Reversed Phase Supports. Methods Mol Biol 1355:31-50
Arias, Franchesca; Arnsten, Julia H; Cunningham, Chinazo O et al. (2016) Neurocognitive, psychiatric, and substance use characteristics in opioid dependent adults. Addict Behav 60:137-43
Carvallo, Loreto; Lopez, Lillie; Che, Fa-Yun et al. (2015) Buprenorphine decreases the CCL2-mediated chemotactic response of monocytes. J Immunol 194:3246-58
Nika, Heinz; Angeletti, Ruth Hogue; Hawke, David H (2014) N-terminal protein characterization by mass spectrometry using combined microscale liquid and solid-phase derivatization. J Biomol Tech 25:77-86
Nika, Heinz; Hawke, David H; Angeletti, Ruth Hogue (2014) N-terminal protein characterization by mass spectrometry after cyanogen bromide cleavage using combined microscale liquid- and solid-phase derivatization. J Biomol Tech 25:19-30
Nika, Heinz; Hawke, David H; Angeletti, Ruth Hogue (2014) C-terminal protein characterization by mass spectrometry: isolation of C-terminal fragments from cyanogen bromide-cleaved protein. J Biomol Tech 25:1-18
Berezniuk, Iryna; Lyons, Peter J; Sironi, Juan J et al. (2013) Cytosolic carboxypeptidase 5 removes ?- and ?-linked glutamates from tubulin. J Biol Chem 288:30445-53
Fleitz, Antoine; Nieves, Edward; Madrid-Aliste, Carlos et al. (2013) Enhanced detection of multiply phosphorylated peptides and identification of their sites of modification. Anal Chem 85:8566-76
Nika, Heinz; Nieves, Edward; Hawke, David H et al. (2013) Phosphopeptide enrichment by covalent chromatography after derivatization of protein digests immobilized on reversed-phase supports. J Biomol Tech 24:154-77
Nika, Heinz; Nieves, Edward; Hawke, David H et al. (2013) Optimization of the ?-elimination/michael addition chemistry on reversed-phase supports for mass spectrometry analysis of O-linked protein modifications. J Biomol Tech 24:132-53

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