Abstract

This application proposes to build an infrastructure for human embryonic stem cell research that will serve both the University of Washington and the Fred Hutchinson Cancer Research Center. Fundamental to this effort will be the establishment of a Human Embryonic Stem Core Laboratory, which will provide a centralized facility for the culture, maintenance, manipulation, and differentiation of human ES cells, and will serve as a resource for support and training of the local research community. Three Pilot Projects will complement the Core Laboratory to establish a foundation for human ES cell research in Seattle. The NIH-approved human ES cell lines to be used are: BG01, BG02, BG03, BG04, ES01, ES02, ES03, ES04, ES06, UC06, WA01, WA09.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM069983-04
Application #
7115349
Study Section
Special Emphasis Panel (ZGM1-CMB-8 (SC))
Program Officer
Zatz, Marion M
Project Start
2003-09-30
Project End
2008-08-31
Budget Start
2006-09-01
Budget End
2008-08-31
Support Year
4
Fiscal Year
2006
Total Cost
$739,664
Indirect Cost

  Institution

Name
University of Washington
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Trowbridge, Jennifer J; Guezguez, Borhane; Moon, Randall T et al. (2010) Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity. Stem Cells 28:1379-89
Ware, Carol B; Wang, Linlin; Mecham, Brigham H et al. (2009) Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells. Cell Stem Cell 4:359-69
Golob, Jonathan L; Paige, Sharon L; Muskheli, Veronica et al. (2008) Chromatin remodeling during mouse and human embryonic stem cell differentiation. Dev Dyn 237:1389-98
Bar, Merav; Wyman, Stacia K; Fritz, Brian R et al. (2008) MicroRNA discovery and profiling in human embryonic stem cells by deep sequencing of small RNA libraries. Stem Cells 26:2496-505
Sampath, Prabha; Pritchard, David K; Pabon, Lil et al. (2008) A hierarchical network controls protein translation during murine embryonic stem cell self-renewal and differentiation. Cell Stem Cell 2:448-60
Hall, Lisa L; Byron, Meg; Butler, John et al. (2008) X-inactivation reveals epigenetic anomalies in most hESC but identifies sublines that initiate as expected. J Cell Physiol 216:445-52
Ueno, Shuichi; Weidinger, Gilbert; Osugi, Tomoaki et al. (2007) Biphasic role for Wnt/beta-catenin signaling in cardiac specification in zebrafish and embryonic stem cells. Proc Natl Acad Sci U S A 104:9685-90
Laflamme, Michael A; Chen, Kent Y; Naumova, Anna V et al. (2007) Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts. Nat Biotechnol 25:1015-24
Trowbridge, Jennifer J; Xenocostas, Anargyros; Moon, Randall T et al. (2006) Glycogen synthase kinase-3 is an in vivo regulator of hematopoietic stem cell repopulation. Nat Med 12:89-98
Lamba, Deepak A; Karl, Mike O; Ware, Carol B et al. (2006) Efficient generation of retinal progenitor cells from human embryonic stem cells. Proc Natl Acad Sci U S A 103:12769-74

Showing the most recent 10 out of 16 publications