The overall goals of the Structural Biology (SB) core are to apply structural techniques to the analysis of important biological macromolecules and their signaling networks, to provide basic knowledge of disease mechanisms, drive research and direct the synthesis of novel therapeutics. When diffraction quality crystals can be grown then atomic images of the arrangement of amino acid side chains in three dimensions can be obtained. These images give the atomic detail needed to visualize the active sites of enzymes, see the DNA binding sites of transcription factors and view the protein-protein interactions of signaling molecules. Function can be understood through determination of atomic structures. In the absence of crystals, the molecular envelopes of macromolecular complexes, individual proteins and their gross conformational changes upon ligand binding can be determined using small angle X-ray scattering (SAXS). Posttranslational modifications (PTMs) alter protein structure and are important for cell signaling. PTMs can be studied using western analysis and isoelectric focusing (IEF). The SB core provides nanocapillary electrophoresis (nanoELP) immunoassays for the characterization of PTMs. When combined these data allow researchers to learn how to modify the structure/function relationships of macromolecules; a key component to developing specific therapies without side effects.
The Structural Biology (SB) core provides training and services that assist researchers at all levels in the Nebraska INBRE network to apply structural techniques to their biological macromolecules of interest. Assistance is provided in the characterization and preparation of samples. Then low and atomic resolution three-dimensional X-ray imaging techniques can be applied to a variety of biomedical problems that range from understanding the mechanisms of disease to visualizing how therapeutics interact with macromolecules.
|Wehrkamp, Cody J; Natarajan, Sathish Kumar; Mohr, Ashley M et al. (2018) miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family. RNA Biol 15:391-403|
|Lopez, Wilfredo; Page, Alexis M; Carlson, Darby J et al. (2018) Analysis of immune-related genes during Nora virus infection of Drosophila melanogaster using next generation sequencing. AIMS Microbiol 4:123-139|
|Fletcher, James T; Sobczyk, Jill M; Gwazdacz, Sarah C et al. (2018) Antimicrobial 1,3,4-trisubstituted-1,2,3-triazolium salts. Bioorg Med Chem Lett 28:3320-3323|
|Kumar, Vinod; Kumar, Virender; Chaudhary, Amit Kumar et al. (2018) Impact of miRNA-mRNA Profiling and Their Correlation on Medulloblastoma Tumorigenesis. Mol Ther Nucleic Acids 12:490-503|
|Spagnol, Gaelle; Trease, Andrew J; Zheng, Li et al. (2018) Connexin43 Carboxyl-Terminal Domain Directly Interacts with ?-Catenin. Int J Mol Sci 19:|
|Leiferman, Amy; Shu, Jiang; Grove, Ryan et al. (2018) A diet defined by its content of bovine milk exosomes and their RNA cargos has moderate effects on gene expression, amino acid profiles and grip strength in skeletal muscle in C57BL/6 mice. J Nutr Biochem 59:123-128|
|Cserhati, Matyas F; Mooter, Mary-Ellen; Peterson, Lauren et al. (2018) Motifome comparison between modern human, Neanderthal and Denisovan. BMC Genomics 19:472|
|Luan, Haitao; Mohapatra, Bhopal; Bielecki, Timothy A et al. (2018) Loss of the Nuclear Pool of Ubiquitin Ligase CHIP/STUB1 in Breast Cancer Unleashes the MZF1-Cathepsin Pro-oncogenic Program. Cancer Res 78:2524-2535|
|Desa, Danielle E; Nichols, Michael G; Smith, Heather Jensen (2018) Aminoglycosides rapidly inhibit NAD(P)H metabolism increasing reactive oxygen species and cochlear cell demise. J Biomed Opt 24:1-14|
|Shaw, Jeff A; Henard, Calvin A; Liu, Lin et al. (2018) Salmonella enterica serovar Typhimurium has three transketolase enzymes contributing to the pentose phosphate pathway. J Biol Chem 293:11271-11282|
Showing the most recent 10 out of 146 publications