The KBRIN-Centralized Core Facility, otherwise known at the Uofl, as the Uofl GG enomics Core Facility, has been operating as a merged INBRE-COBRE operation since 2008, although certain individual components were available separately from either the INBRE or COBRE awards since 2001. Its objectives are to provide state of the art genomic related technologies, including microarray and deep sequencing services to Uofl researchers as well as to investigators in the KBRIN-affiliated institutions and its outreach institutions across the Commonwealth of Kentucky. Its services are centered around the most advanced and comprehensive platforms of high-density oligonucleotide array technologies offered by Affymetrix, Inc. and Agilent, Inc, as well as the next generation sequencing capabilities of the Jon Torrent PGM platform. These platforms allow standardized analyses of RNA or miRNA expression profiles, splice patterns and variations, DNA mutations, and single nucleotide polymorphism profiles. The Facility ensures that all investigators, including clinical faculty and basic science groups, will be able to adopt such state-of-the-art technologies for their investigative goals using clinical specimen, or animal model or cell culture materials. Since its inception, the Facility has experienced a rapid growth in personnel, user numbers and diversity of experimental platforms, allocated space, attendance in training events, and resources for data analysis and mining. It has been an integral part of several funded and/or currently pending individual and multi-investigator Center and Training grant applications, the strongest case for its longevity and its continued utility. The Facility is currently a key component of COBRE 8P20GM103482-10 Center of Biomedical Research Excellence in Molecular Targets (D. Miller, P.l.), COBRE 8P20GM103453-10 Molecular Determinants of Developmental Defects (R. Greene, P.l.), NIGMS- KY-INBRE P20GM103436, KY-IDeA Networks of Biomedical Research Excellence award (N. Cooper, P.l.), and training grant NIEHS T32 ES11564 Uofl EElnvironmental Health Sciences Training Program (D. Hein, P.l.).

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
2P20GM103436-14
Application #
8716031
Study Section
Special Emphasis Panel (ZGM1-TWD-7 (IN))
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-04-30
Support Year
14
Fiscal Year
2014
Total Cost
$35,874
Indirect Cost
$11,958
Name
University of Louisville
Department
Type
DUNS #
057588857
City
Louisville
State
KY
Country
United States
Zip Code
40202
Toh, Su San; Chen, Zehua; Rouchka, Eric C et al. (2017) Pas de deux: An Intricate Dance of Anther Smut and Its Host. G3 (Bethesda) :
Sledge, Samiyyah; Henry, Collin; Borchman, Douglas et al. (2017) Human Meibum Age, Lipid-Lipid Interactions and Lipid Saturation in Meibum from Infants. Int J Mol Sci 18:
Curran, Christine Perdan; Marczinski, Cecile A (2017) Taurine, caffeine, and energy drinks: Reviewing the risks to the adolescent brain. Birth Defects Res 109:1640-1648
Lee Stubbeman, Bobbie; Brown, Clifford J; Yates, Justin R et al. (2017) Early-life risperidone enhances locomotor responses to amphetamine during adulthood. Eur J Pharmacol 812:256-263
Bamji, Sanaya F; Rouchka, Eric; Zhang, Yan et al. (2017) Next generation sequencing analysis of soy glyceollins and 17-? estradiol: Effects on transcript abundance in the female mouse brain. Mol Cell Endocrinol :
Klyczek, Karen K; Bonilla, J Alfred; Jacobs-Sera, Deborah et al. (2017) Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages. PLoS One 12:e0180517
Muehlenkamp, Jennifer J; Brausch, Amy M; Washburn, Jason J (2017) How much is enough? Examining frequency criteria for NSSI disorder in adolescent inpatients. J Consult Clin Psychol 85:611-619
Yates, Justin R; Rogers, Katherine K; Gunkel, Benjamin T et al. (2017) Effects of Group I metabotropic glutamate receptor antagonists on sensitivity to reinforcer magnitude and delayed reinforcement in a delay-discounting task in rats: Contribution of delay presentation order. Behav Brain Res 322:29-33
Yao, Sen; Flight, Robert M; Rouchka, Eric C et al. (2017) Perspectives and expectations in structural bioinformatics of metalloproteins. Proteins 85:938-944
Yao, Sen; Flight, Robert M; Rouchka, Eric C et al. (2017) Aberrant coordination geometries discovered in the most abundant metalloproteins. Proteins 85:885-907

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